花生序列相关扩增多态性(SRAP)标记的研究(英文)  

The Sequence-related Amplification Polymorphisms in Cultivated Peanut (Arachis hypogaeaL.)

作　　者：王传堂[1] 张建成[1] 杨新道[1] 徐建芝[1] 禹山林[1] 

机构地区：[1]山东省花生研究所

出　　处：《花生学报》2005年第3期11-15,共5页Journal of Peanut Science

基　　金：科技部973前期项目(2002ccc03200);国家自然科学基金项目(30300224)

摘　　要：研究了分属3个市场型的10个中国花生栽培种的序列相关扩增多态性(SRAP).结果表明,SARP技术可以揭示花生栽培种的遗传差异.在所试验的51对SRAP 引物中,2对只获得了单态性条带,其余49对总共产生了1087 条带,其中 503 条(46.27%) 为多态性带, 每对引物组合平均获得22.18条总带、10.26 条多态性带. 在这49对引物组合中,总带数和多态性条带的数目分别为7～63 和 2～32 条. 10个花生栽培种间的简单匹配相似系数为0.7712～0.9250不等,根据SRAP指纹图谱进行聚类分析可将这10个品种分为 4 类.另外一项采用作图亲本24-3和B4的研究中, 40对SRAP引物共计获得了160条多态性带.本研究为花生品种鉴定和遗传研究提供了新的DNA分子标记技术手段.SRAP assay was conducted using 10 peanut cultivars from China of 3 market types and 51 SRAP primer combinations to evaluate the utility of SARP in peanut. Of the primer pairs tested, 2 produced monomorphic bands only, and 49 totally produced 1087 bands, among which 503 （46.27%） were polymorphic, with an average of 10.26 polymorphic bands and 22.18 bands per primer combination. The total number of bands and polymorphic bands in the 49 primer combinations ranged from 7～63 and 2～32 respectively. The simple matching coefficient of the peanut varieties ranged from 0.7712 to 0.9250. The varieties may be grouped into 4 categories based on cluster analysis of the SRAP fingerprinting data. In a separate study on SARP using 40 primer pairs, 160 polymorphic bands were detected in 24-3 and B4, the parental genotypes for mapping. This study provided an additional marker system for peanut variety identification and genetic research.

关 键 词：花生 栽培种 SRAP图谱 

分 类 号：S565.203[农业科学—作物学]