花生白藜芦醇合酶基因的DNA克隆及序列分析  

Cloning and Sequence Analysis of Resveratrol Synthase DNA from Peanut(Arachis hypogaea L.)

作　　者：王冰梅[1] 潘海芳[1] 叶冰莹[1] 黄骐 朱锦懋[1] 陈如凯[1] 陈由强[1] 

机构地区：[1]福建师范大学生物工程学院,福建福州350007 [2]福州市农业科学研究所,福建福州350018

出　　处：《福建师范大学学报（自然科学版）》2005年第3期56-60,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：国家自然科学基金资助项目(30371177);福建省自然科学基金资助项目(B0310004);福建省科技厅基金资助项目(K04030);福建省教育厅基金资助项目(03WC394);福建省教育厅基金资助项目(JA03171)

摘　　要：白藜芦醇合酶(resveratrol synthase,RS)是合成白藜芦醇的关键酶.通过PCR方法从花生基因组DNA中分离出白藜芦醇合酶基因,将其克隆到pMD18-T和pBluescript Ⅱ KS(+)载体并测序,获得全长1 537 bp的片段.序列分析表明,此序列含有2个外显子和1个内含子,编码区由389个氨基酸组成,其相对分子质量为42 800.该白藜芦醇合酶氨基酸368～378位点上存在芪合酶家族的特征位点GVLFGFGPGLT.Resveratrol synthase is a key enzyme in the synthasis of resveratrol. A full-length RS gene DNA of 1 537 bp was amplified from peanut by PCR method, which was cloned into the vector pMD18-T and pBluescript II KS （ ＋ ）. Analysis of the nucleotide sequence indicated that the sequence contained two extrons and one intron, and the reading frame encoded a protein of 389 amino acid whose molecular weight is 42 800. The amino acid sequence GVLFGFGPGLT was found at position 368-378 which is the family signature sequence for stilbene svnthase.

关 键 词：花生 白藜芦醇合酶 基因克隆 序列分析 

分 类 号：Q943[生物学—植物学]