双层类脂膜非标记乙型肝炎表面抗原免疫传感器  

Non-labeled HBsAg Immunosensor Based on Bilayer Lipid Membrane

作　　者：谭学才[1] 李荫[2] 梁汝萍[2] 罗利军[2] 蔡沛祥[2] 邹小勇[2] 

机构地区：[1]广西民族学院化学与生态工程学院,广西南宁530006 [2]中山大学化学与化学工程学院,广东广州510275

出　　处：《分析测试学报》2005年第5期1-5,共5页Journal of Instrumental Analysis

基　　金：国家自然科学基金资助项目(20475068);广东省自然科学基金资助项目(031577);广西壮族自治区自然科学基金资助项目(0342018)

摘　　要：采用固体铂盘电极支撑的双层类脂膜(Pt-BLM)技术固定乙肝表面抗体(HBsAb),研制成Pt-BLM-HBsAb电位型非标记免疫传感器,用于检测人血清中乙肝表面抗原(HBsAg).对影响构筑BLM的各种因素进行了研究,用循环伏安法对pt-BLM进行了表征.传感器对HBsAg呈Nernst响应,其线性范围为2～320μg/L,线性回归方程为△V=-1.09+32.4lg ρ(HBsAg),线性相关系数r=0.999 8.该传感器响应迅速,灵敏度高,稳定性及选择性好,于4℃干态保存4周,其响应信号基本不变.将其用于临床血清检验,结果满意.A new type of non-labeled potentiometric immunosensor（Pt - BLM - HBsAb） for the determination of hepatitis B surface antigen （HBsAg） was developed by immobilizing hepatitis B surface antibody （HBsAb） in the bilayer lipid membrane（BLM）, which was supported hy the platinum disk electrode（Pt- BLM）. Effects of some experimental conditions such as the ratio of cholesterol and lecithin, species of the solvent, and the added quantum of HBsAb etc. on the potential response of the immurnosensor were investigated. The immunosensor was characterized by cyclic vohammetry. The response potential of the inununosensor is linear with p （HBsAg）, over a concentration range of 2- 320μg/L with a correlation coefficient of 0. 999 8 and a linear regression equation of ΔV = - 1.09 ＋ 32.4 lgρ（HBsAg） . The immunosensor has a fast response time, high sensitivity, good selectivity and stability of about four weeks when storing a dry, state at 4℃. This method has been used to detennine the real serum samples with satisfactory results.

关 键 词：双层类脂膜 乙肝表面抗体 乙肝表面抗原 非标记免疫传感器 

分 类 号：O657.1[理学—分析化学]