聚己内酯的细胞毒性和动物急性毒性实验  

Study on cytotoxicity and animal acute toxicity of poly-ε-caprolactone

作　　者：蒋青锋[1] 陈建海[1] 陈志良[1] 武金宝[1] 阎玺庆[1] 包杰[1] 

机构地区：[1]广州南方医院,广东广州510515

出　　处：《中国医院药学杂志》2005年第9期798-800,共3页Chinese Journal of Hospital Pharmacy

基　　金：广东省科技计划项目(编号:2002C30103)

摘　　要：目的:研究聚己内酯(PCL)对L929细胞生长抑制作用的影响及其对小鼠急性毒性的作用。方法:采用四甲基偶氮唑盐(MTT)比色法和吖啶橙荧光染色法研究不同浓度的PCL浸提液对L929细胞生长抑制的影响;选用清洁级昆明种小鼠进行了动物急性毒性实验研究。结果:不同浓度的PCL浸提液对L929细胞在2,4,7d显示毒性级别分别为1级,0级,0级,PCL各处理组细胞与正常对照组细胞的凋亡指数差异无显著性。日给药量20g·kg-1时,给药组小鼠行为活动正常,与对照组小鼠的体重相比差异无显著性。结论:高分子材料PCL符合生物体应用的基本要求,安全无毒有较佳的生物相容性。25%PCL混悬液小鼠灌胃给药,对小鼠基本无毒。OBJECTIVE To investigate the growth inhibition of poly-ε-caprolactone（PCL） on L 929 cell and its acute animal toxicity on mice. METHODS The influence of growth inhibition of PCL in different concentration on L929 cell was studied by MTT colorimetric assay and acridine orange fluorescence staining. The animal acute toxicity of PCL was conducted with KM mice in CL grade. RESULTS The toxic grade of PCL in different concentration on L 929 cell was shown in one-, zero-and zero-grade on the 2^nd day, 4th day and 7th day respectively. Also there had no different significance between PCL groups and control group in apoptosis index. After oral administration of PCL in 20 g. kg^-1, no significant difference occurred between the treated and untreated groups of mice. CONCLUSION The nontoxic of PCL toward L929 cell indicated that PCL had excellent biocompatibility. Also no acute animal toxicity on mice was found after oral administration.

关 键 词：聚己内酯 MTT法 吖啶橙荧光染色法 动物急性毒性 凋亡指数 

分 类 号：R963[医药卫生—微生物与生化药学]