表达乙型脑炎病毒抗原的非复制型重组痘苗病毒能在小鼠中诱发免疫保护  

Non-Replicating Vaccinia Recombinant Expressed Antigens of Japanese Encephalitis Virus Can Induce Protective Immunity in Mice

作　　者：王文玲[1] 陆振华 辛伟[1] 李仁清[1] 张相民[1] 邓瑶[1] 陆柔剑[1] 梁国栋[1] 阮力[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《病毒学报》2005年第5期370-375,共6页Chinese Journal of Virology

基　　金：国家863项目(编号:863-102-07-02-01)

摘　　要：使用2×107PFU乙脑病毒野毒株GSS抗原preM-E-NS1-NS2a的非复制型重组痘苗病毒NTV-JEV免疫3周龄BALB/c小鼠,小鼠体内乙脑病毒特异性抗体滴度1∶71,IgG2a/IgG1为1.5,中和抗体滴度为1∶8,免疫组小鼠产生特异性补体介导的细胞杀伤作用。以10 LD50乙脑P3株病毒对小鼠进行了脑内攻击,NTV-JEV免疫组小鼠存活率为100%,与乙脑减毒活疫苗SA14-14-2免疫组存活率相同。100 LD50P3株病毒攻击后NTV-JEV免疫组存活率28.6%,与SA14-14-2组免疫效果无明显差别。存活小鼠体内乙脑病毒特异性抗体升高为1∶179,IgG2a/IgG1比值为1.9,中和抗体大于1∶16。以30 LD50GSS株病毒进行攻击后,NTV-JEV免疫组和SA14-14-2组保护率均为8.3%。上述结果表明,NTV-JEV与乙脑减毒活疫苗SA14-14-2株具有相近的保护力,NTV-JEV不仅可以保护小鼠抵抗同株病毒GSS的攻击,而且可以抵抗异株病毒P3的攻击。Four groups of 3-week-old BalB/c mice were immunized twice by intramuscular （i. m. ） injection with 2 × 10^7 PFU/mouse of non-replicating vaccinia recombinant NTV-JEV which expresses the preM-E-NS1-NS2a antigens of Japanese encephalitis virus（JEV）. Three weeks after the second immunization, the mice produced JEV-specific antibody with total antibody titer of 1 ： 71, IgG2a/IgG1 ratio of 1.5 and neutralizing antibody of 1 ： 8. Antibody-dependent complement-mediated cytotoxic activity was detected only in NTV-JEV immunized mice but not in mice immunized with attenuated live vaccine SA14-14-2 . After challenged with 10 LD50 JEV P3 strain, 100 % of the mice immunized with either NTV-JEV or attenuated live vaccine SA14-14-2 were survived. After challenged with 100 LD50 P3 strain, only 28.6% of the mice in NTV-JEV immunized group were survived, that was similar to that in attenuated live vaccine SA14-14-2 immunized group. JEV-specific antibody titer in the survived mice increased to 1 ： 179, the ratio of IgG2a/IgG1 was 1.9, and the neutralizing antibody titer exceeded 1 ： 16. When challenged with 30 LDs0 of GSS strain, both the groups immunized with NTV-JEV and attenuated vaccine SA14-14-2 had the same survival ratio of 8.3 %. All the data above showed that NTV- JEV has the similar protective efficacy with that of the attenuated live vaccine SA14-14-2. NTV-JEV induced protective immunity against challenge not only with the homologous strain GSS but also with the heterologous strain P3.

关 键 词：非复制型痘苗病毒 乙脑疫苗 免疫学 

分 类 号：R373[医药卫生—病原生物学] Q78[医药卫生—基础医学]