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作 者:赵艳岭[1] 刘志强[1] 邢红华[1] 赵强 华金荣[3]
机构地区:[1]河南省科学院生物研究所,河南郑州450008 [2]郑州中多电子计算机网络公司,河南郑州450000 [3]河南省中医院,河南郑州450003
出 处:《河南科学》2005年第5期689-691,共3页Henan Science
摘 要:香石竹试管苗玻璃化现象给试管苗的应用带来困难,采用强光照10000—20000 LX,在培养基中提高蔗糖和琼脂浓度,降低激素用量,对克服香石竹试管苗玻璃化有明显效果.青霉素对克服香石竹试管苗玻璃化无明显效果,但对生根有促进作用,其作用机制有待进一步研究.The phenomenon of Vitrification phenomenon is a big obstruction in the propagantion of carnation by tissue culture, the effective way to decrease the vitrification phenomenon is to increase the concentration of sucrose and agar in the medium, to use strong luminosity of 10000-20000 LX, and to decrease the concentration of hormone. There is not evident effect of penicillin to control the vitrification shoots, but penicillin can stimulate the growth of carnation plantlet root in the medium, the mechanism of penicillin function to the root is not clear and it wll be studied further.
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