青紫薯色素对β淀粉样肽诱导神经细胞毒性的作用  

EFFECT OF SWEET-POTATO ANTHOC YANINS ON CYTOTOXICITY OF PC-12 CELL INDUCED BY AMYLOID β PROTEIN

作　　者：万瑞香[1] 孟祥军 张海平[1] 初晓[3] 韩志武[1] 刘涵云[1] 李润生 王春波[5] 

机构地区：[1]青岛大学医学院附属医院药品采购管理办公室,山东青岛266003 [2]青岛市第七人民医院心理咨询科 [3]青岛市市立医院药剂科 [4]青岛市农业技术推广站 [5]青岛大学医学院药理学教研室

出　　处：《青岛大学医学院学报》2005年第4期286-288,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：青岛市科技局科研基金资助项目(03-2-JZ-03);山东省教育厅科研基金资助项目(J04E17)

摘　　要：①目的探讨青紫薯色素对β淀粉样肽诱导神经细胞毒性的保护作用及其机制。②方法建立β淀粉样肽诱导的PC-12神经细胞毒性病理模型,MTT法测定青紫薯色素对PC-12神经细胞增殖活性的影响,流式细胞仪检测PC-12神经细胞的凋亡率、线粒体膜电位、细胞内Ca2+浓度和Caspase-3的活性。③结果青紫薯色素可以剂量依赖性地提高PC-12细胞的增殖活性,降低β淀粉样肽诱导的PC-12细胞凋亡率,减轻线粒体膜的损伤,降低细胞内钙离子的浓度,抑制Caspase-3的活化。④结论青紫薯色素通过抑制Caspase-3的活化,减轻线粒体膜的损伤,降低细胞内Ca2+的浓度,起到减轻β淀粉样肽诱导的PC-12细胞损伤的作用。Objective To investigate the effect of sweet-potato anthocyanins on cytotoxicity of PC-12 cells induced by amyloid β（Aβ） protein. Methods A pathological model of PC-12 cell cytotoxicity induced by At3 was established. MTT was applied to measure the effect of sweet-potato anthocyanins on PC-12 cell proliferation ; flow cytometry for analysis of the cell viability, apoptosis, activity of caspase-3, and mitochondria membrane potential, and intracellular Ca^2＋ in PC-12 cells. Results The anthocyanins can dose-dependently increase the PC-12 cell viability, decrease the apoptosis of the cells, improve the mitochondria membrane potential,and decrease the concentration of intracellular Ca^2＋ , and inhibit the activation of caspase-3. Conclusion The protective effect of sweet-potato anthocyanins on PC-12 cell injury induced by Aβ can be reached by ways of inhibition of caspase-3 activation, improvement of mitochondria membrane potential, and a decrease of the concentration of intracellular Ca^2＋.

关 键 词：青紫薯色素 Β淀粉样肽 PC-12细胞 细胞凋亡 

分 类 号：R285[医药卫生—中药学]