纳米材料对细胞IGF-ⅠmRNA表达水平的影响  

EFFECTS OF NANOMATERIAL O N IGF-ⅠmRNA EXPRESSION IN CELLS

作　　者：董辰方[1] 强新[1] 丁守怡[1] 马建伟[1] 

机构地区：[1]青岛大学医学院微生物学教研室,山东青岛266021

出　　处：《青岛大学医学院学报》2005年第4期292-293,296,共3页Acta Academiae Medicinae Qingdao Universitatis

基　　金：山东省自然科学基金重点资助项目(Y2001F04)

摘　　要：①目的探讨纳米材料对胰岛素样生长因子Ⅰ(IGF-Ⅰ) mRNA表达的影响.②方法通过原位杂交、斑点杂交及定量分析技术分别检测银系纳米材料组、稀土纳米材料组与对照组WI-38及Hela细胞IGF-Ⅰ mRNA表达.③结果银系纳米材料组、稀土纳米材料组与对照组的细胞均有IGF-Ⅰ mRNA杂交阳性信号表达.膜上斑点杂交经吸光度扫描表明,WI-38细胞及Hela细胞银系纳米材料组吸光度值与对照组相比差异均无显著性(F=12.4～31.2,tD=1.2～1.8,P>0.05);而稀土纳米材料组吸光度值较对照组均显著性降低(tD=3.2～4.6,P<0.01).④结论银系纳米材料对IGF-Ⅰ mRNA的表达无影响;而稀土纳米材料对IGF-Ⅰ mRNA的表达有较强的抑制作用,在一定程度上影响了其在医学上的应用.Objective To evaluate the effects of nanomaterial on the expression of IGF-Ⅰ mRNA in cells in vitro. Methods The expressions of IGF- Ⅰ mRNA of WI-38 and Hela cell lines in Silver nanomaterial group, rare-earth nanomaterial group and control group were tested by in situ hybridization, dot hybridization and quantitative analysis technique. Results In situ hybridization showed that there was hybridization signal of IGF-Ⅰ mRNA in all groups. Dot hybridization showed that there was no significant difference between the expression of IGF-Ⅰ mRNA in WI-38 and Hela cells in the silver nanomaterial group and that in the control group （F= 12.4-31.2, tD = 1. 2-1. 8, P〉0.05）. But the expression of IGF-Ⅰ mRNA in WI-38 and Hela cells in the rare-earth nanomaterial group was lower than that in the control group （tD=3. 2-4. 6,P〈0.01）. Conclusion Silver nanomaterials has no effects on the expression of IGF-Ⅰ mRNA. Rare-earth nanomaterials can inhibit the expression of IGF-Ⅰ mRNA, which limits its medical application to some extent.

关 键 词：纳米材料 胰岛素样生长因子Ⅰ 原位杂交 斑点杂交 

分 类 号：R318.08[医药卫生—生物医学工程]