中药含药血清对大鼠肝细胞膜ATP酶活性的影响  

The Effect of Herb serum to the Activity of Mice Liver Cell Membrane

作　　者：杨秀竹[1] 李东华[1] 张西波[1] 崔乃强[1] 

机构地区：[1]天津市南开医院,天津300100

出　　处：《中医药学刊》2005年第9期1605-1607,共3页Study Journal of Traditional Chinese Medicine

基　　金：天津市科委课题

摘　　要：目的:观察清热利湿中药对肝细胞膜ATP酶活性的影响。探讨其防治结石形成的机理。方法:采用肝脏原位灌注消化法体外培养大鼠肝细胞,以及血清药理学方法,测定肝细胞膜Ca2+-Mg2+-ATPase、Na+-K+-ATPase的活性。结果:10倍量和20倍量载药(清热利湿中药)血清能提高炎性细胞因子刺激后Ca2+-Mg2+-ATPase、Na+-K+-ATPase活性。结论:清热利湿中药可能是通过提高肝细胞膜ATP酶活性,减轻细胞内钙超载,降低胆汁的成石倾向。Objective ：To observe the effect of QingReLiShi herbs to the activity of ATPase of liver cell membrane, to evaluate the pathogenesis of preventing the formation of pigment stones. Methods ： We adopt the rat hepatic cells by using primary lives infusion method and serum pharmacology method, determine th activity of Ca^2＋ - Mg^2＋ - ATPase and Na^＋ - K^＋ -ATPase of liver cell membrane. Results：lO times and 20 times dose herb serum can improve the activity of Ca^2＋ - Mg^2＋ - ATPase and Na^＋ - K^＋ - ATPase after the incetive of the inflammatory factors. Conclusion ：QingReLiShi herbs decreased the tendency of glasstone by increasing the activity of ATPase of liver cell membrance, relieving calcium overload in cells possibly.

关 键 词：清热利湿中药 Ca^2+-Mg^2+-ATPase NA^+-K^+-ATPASE 炎性细胞因子 

分 类 号：R969[医药卫生—药理学]