应激对大鼠心肌线粒体型硫氧还蛋白还原酶表达的影响  

Effect of oxidative stress on expression of mitochondrial thioredoxin reductase in myocardia in rats

作　　者：邹向晖[1] 邹宁[2] 周令望[2] 于维汉[2] 

机构地区：[1]哈尔滨医科大学生理学教研室,150086 [2]中国疾病预防控制中心地方病控制中心克山病研究所

出　　处：《中国地方病学杂志》2005年第5期498-499,共2页Chinese Jouranl of Endemiology

基　　金：黑龙江省教育厅科学技术资助项目(10531101)

摘　　要：目的探讨线粒体型硫氧还蛋白还原酶(mitochondrialthioredoxinreductase,TR2)在大鼠心肌抗氧化应激反应中的作用。方法将Wistar雄性大鼠分成非应激组和应激组,应激组大鼠在处死前一天在冰水浴中泳动5min,采用Northernblot和Westernblot检测大鼠心肌线粒体型硫氧还蛋白还原酶和蛋白的表达。结果应激组大鼠心肌中线粒体型硫氧还蛋白还原酶基因和蛋白表达均高于非应激组。结论线粒体型硫氧还蛋白还原酶基因和蛋白表达的增加有利于大鼠心肌抗氧化损伤。Objective To explore the effect of oxidative stress on expression of mitochondrial thioredoxin reduetase in myoeardia in rats. Methods The male Wistar rats were randomly divided into control and stress group. The stress group rats swam in iced water for five minutes before sacrifice. The expression of mitoehondrial thioredoxin reduetase and protein were detected in heart with Northern blot and Western blot methods. Results The expressions of the protein and gene of mitoehondrial thioredoxin reduetase in stress group were higher than those of control group. Conclusions The increased expression of mitoehondria thioredoxin reduetase may protect the heart against oxidative stress injury.

关 键 词：氧化性应激 大鼠 心肌 硫氧还蛋白还原酶 

分 类 号：Q593[生物学—生物化学]