对5个S180克隆细胞株RAPD特征的研究  

RAPD Characterization of 5 Clonal Cell Strains from S180

作　　者：贺文[1] 张焕玲[1] 王俊霞[1] 刘福英[1] 

机构地区：[1]河北医科大学实验动物学部,石家庄050017

出　　处：《中国实验动物学报》2005年第3期173-175,共3页Acta Laboratorium Animalis Scientia Sinica

基　　金：河北省科学技术厅基金项目(03276196D-48)。

摘　　要：目的运用随机扩增多态性DNA(RAPD)技术建立S180的5个克隆细胞株的特异性图谱。方法运用23条引物对S180-S2D9、S180-S2D7、S180-S1F11、S180-S1H10、S180-S1B115个克隆细胞株的基因组DNA进行RAPD-PCR扩增,以琼脂糖电泳观察结果。结果筛选出3条在各克隆株扩增产物间有差异的引物。结论5个S180克隆细胞株的RAPD特征有明显区别。Objective To set up differential specific maps of 5 clonal cell strains from S180 with RAPD. Methods The Genomic DNA of the 5 clonal strains from S180 including S2D9, S2D7, S1F11, S1H10 and S1B11 was amplified with RAPD-PCR using 23 single primers. The PCR results were assessed by electrophoresis on agarose gel. Results 3 of the 23 single primers were found to be different between amplified products from different clonal strains. Conclusion RAPD characteristics of the 5 clonal cell strains from S180 are apparently different.

关 键 词：随机扩增多态DNA技术 S180 克隆细胞 DNA引物 

分 类 号：Q813[生物学—生物工程]