Ru(bipy)_3^(2+)-Ce(Ⅳ)化学发光体系检测速尿的研究  

Detection of Furosemide with Tris-(2,2′-bipyridyl)ruthenium- Ce(Ⅳ) Chemiluminescence System

作　　者：张韶虹[1] 郗娟[1] 何治柯[1] 

机构地区：[1]武汉大学化学与分子科学学院

出　　处：《分析化学》2005年第9期1269-1271,共3页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20275028);国家高技术研究发展计划(863)项目(No.2002AA2Z2004)

摘　　要：基于速尿对三(2,2′-联吡啶)钌(Ⅱ)[Ru(bipy)32+]-Ce(Ⅳ)化学发光体系发光的增强作用,建立了一种化学发光直接检测速尿的新方法。在优化的实验条件下,该方法的线性范围和检出限分别为5.0×10-3~1.5mg/L和4.0×10-3mg/L,对含速尿0.50mg/L的溶液平行测定11次,相对标准偏差(RSD)为5.0%。将其应用于呋塞米片剂样品中速尿含量的测定,结果满意。Furosemide is a kind of forbidden medicines for sportsmen. It was observed that furosemide could greatly enhance the chemiluminescence （CL） intensity of tris-（ 2, 2＇-bipyridyl ） ruthenium （ Ⅱ ） （Ru（bipy）3^2＋）-Ce（Ⅳ） system, based on which, a novel CL method was developed for detecting it. Under the optimum experimental conditions of 20 mg/L Ru（bipy）3Br2, 0. 16 mmol/L Ce（ IV ） and 16 mmol/L n2so4 , the linear range and detection limit of the proposed method were 5.0×10^-3-1.5 mg/L and 4.0×10^-3 mg/L, respectively. The relative standard deviation of determining 11 solutions containing 0.50 mg/L furosemide was 5.0%. The proposed CL method has been applied to detect furosemide in the troche with satisfactory results.

关 键 词：速尿 化学发光 三(2 2’-联吡啶)钌(Ⅱ) 化学发光体系 Ce(Ⅳ) 直接检测 相对标准偏差 增强作用 实验条件 线性范围 

分 类 号：O657.3[理学—分析化学] X132[理学—化学]