柱前衍生化高效液相色谱法分析当归多糖的单糖组成  

Analysis of Monosaccharide Composition in Angelica Polysaccharides by Precolumn Derivatization High Performance Liquid Chromatography

作　　者：杨兴斌[1] 赵燕[2] 周四元[1] 刘莉[1] 王海芳[1] 梅其炳[1] 

机构地区：[1]第四军医大学药理学教研室,西安710032 [2]第四军医大学化学教研室,西安710032

出　　处：《分析化学》2005年第9期1287-1290,共4页Chinese Journal of Analytical Chemistry

基　　金：陕西省科技攻关项目(No2002K10-G2);西安市科技攻关项目(NoSF200224)

摘　　要：采用1-苯基-3-甲基-5-吡唑啉酮(PMP)柱前衍生化反相高效液相色谱(HPLC)法,建立了9种常见单糖的分离模式,并成功地用于当归多糖的单糖组成分析,具有良好的重复性。结果表明,当归多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖及阿拉伯糖7种单糖组成,其摩尔比为0.57∶1.00∶0.13∶3·06∶8.16∶7.17∶12.69。该HPLC方法是简单、快速、灵敏、方便的分析技术,可用于当归多糖的质量控制。The reversed-phase high performance liquid chromatographic （HPLC） method of precolumn-derivatization with 1-phenyl-3-methyl-5-pyrazolone （PMP）. was developed to separate the traditional nine monosaccharides and detect the monosaccharide composition of Angelica polysaccharides with good reproducibility. In this study, it was found that sulfuric acid and trifluoroacetic acid possessed the same ability for hydrolyzing Angelica polysaccharides and the component monosaccharides were completely released from the polysaccha- rides until hydrolyzing for 8 h, and the whole drying processes of the acid hydrolysates and their PMP-derivatives could be omitted. The results of HPLC analysis showed that Angelica polysaccharides consisted of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabonose with the molar ratio of 0.57：1.00：0.13：3.06：8.16： 7.17：12.7. The HPLC method is simple, rapid, sensitive, convenient and can be applied to the quality control of Angelica polysaccharides.

关 键 词：当归多糖 单糖组成 衍生化 高效液相色谱 柱前衍生化 高效液相色谱法 1-苯基-3-甲基-5-吡唑啉酮 多糖 当归 高效液相色谱(HPLC) 

分 类 号：O657.7[理学—分析化学]