聚合酶链式反应微芯片系统用于微量生物样品的快速扩增检测  

An Integrated Polymerase Chain Reaction Microchip System for Deoxyribonucleic Acid Sample Quick Amplification

作　　者：邹志青[1] 陈飞[1] 周洪波[1] 金庆辉[1] 杨梦苏[1] 赵建龙[1] 

机构地区：[1]中国科学院上海微系统与信息技术研究所生物芯片实验室,上海200050

出　　处：《分析化学》2005年第9期1354-1357,共4页Chinese Journal of Analytical Chemistry

基　　金：中国科学院生物科学与生物技术研究特别支持(No.STZ-00-03);中国科学院知识创新工程(No.KSCX-06)资助项目

摘　　要：基于MEMS微加工技术设计和制作了一种集成微加热器和温度传感器的聚合酶链式反应(PCR)微芯片。PCR微芯片结构通过有限元模拟验证分析。该芯片在PCR扩增过程中具有良好的制热效率和热传递均匀性。在微型温度控制电路装置下进行热循环反应,芯片的温度起伏小于1℃/s,升降温速度分别达到5℃/s和3℃/s,30个热循环耗时30min。此系统已经用于GUS基因的扩增检测,获得了良好的结果,极大的缩短了热循环的时间,可用于微量生物样品的快速扩增检测。A polymerase chain reaction （PCR） microchip integrated with microheaters and temperature sensors has been designed and fabricated based on micro electromechanic system （MEMS） techniques. The structure of the proposed device is verified by using Finite Element Analysis （FEA） to optimize the thermal performance. The PCR microchip has high heat generation efficiency and heat transfer uniformity in the deoxyribonucleic acid （DNA） amplification. By combining peripheral thermal control system, a temperature fluctuation of ±1℃, a heating rate of 5℃/s, and a cooling rate of 3℃/s have been achieved, and 30 thermal cycles have been finished in 30 minutes. The system has been used for deoxyribonucleic acid （DNA） amplification of GUS gene and good results were obtained from the experiments with much less thermal time. It can be used for DNA sample quick amplification.

关 键 词：聚合酶链式反应微芯片 微加热器和温度传感器 聚合酶链式反应 芯片系统 快速扩增 生物样品 检测 微量 温度传感器 PCR扩增 

分 类 号：Q503[生物学—生物化学]