RP-HPLC测定贯叶连翘中的金丝桃素含量  

Determination of Hypericin from Extraction of Hypericum perforatum L by RP-HPLC

作　　者：崔颖[1] 梁剑平[1] 王学红[1] 王选慧[2] 祝艳华[2] 尚若锋[1] 华兰英[1] 赵晓虹[2] 孙瑶[1] 

机构地区：[1]中国农业科学院兰州畜牧与兽药研究所农业部新兽药工程重点开放实验室,甘肃兰州730050 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《动物医学进展》2005年第9期43-45,共3页Progress In Veterinary Medicine

基　　金："十五"国家科技攻关项目(2002BA514A-17-07)

摘　　要：采用反相高效液相色谱法测定了贯叶连翘提取物金丝桃素浸膏中金丝桃素的含量。用Kromasil C18(5μm,250×4.6 mm)色谱柱为固定相,乙腈-水(140∶10)为流动相,流速为1 mL/min,检测波长为284 nm。结果金丝桃素的含量为0.914%,平均回收率为96.76%,日内精密度RSD为2.67%,日间精密度RSD为3.65%。A method of reversed-phase high performance liquid chromatography （RP-HPLC） for determination of hypericin in the extractum hypericin of the extraction of hypericum perforatum Linn. The compounds was separated on Kromasil C18 RP-HPLC colum and quantified by measuring the UV absorbance at 284 nm. A mixture of acetonitrile and water （140 ： 10） was used as the mobile phase with a flow rate of 1 mL/min. The hypericin content that was determined is 0. 914%. The average recovery was 96.76%. The coefficient of variation of with in day and day to day precision was 2.67% and 3.65%. seneratelv.

关 键 词：反相高效液相色谱法 金丝桃素 含量测定 

分 类 号：S853.731[农业科学—临床兽医学]