用激光衍射法测量网织红细胞膜剪切弱性模量(E)和表面粘度  

Study on the Membrane Shear Elastic Modulus and Viscosity of Mouse Reticulocytes

作　　者：王金华[1] 喀蔚波[1] 孙大公[1] 文宗曜[1] 

机构地区：[1]北京大学医学部基础医学院物理教研室

出　　处：《中国生物医学工程学报》2005年第4期468-471,476,共5页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金(39830110);博士点基金资助

摘　　要：按Bishop方法,在小鼠血液里诱导生成大量网织红细胞,然后提取网织红细胞。用激光衍射法测得小变形指数(DI)d和变形恢复过程(即松驰过程)中细胞变形恢复到最大值(DI)max一半的时间t0.5(变形恢复半时间),将测得结果分别代入细胞膜的膜剪切弹性模量(E)公式和表面粘度(μm)公式,计算出网织红细胞和成熟红细胞膜的膜剪切弹性模量(E)和表面粘度(μm),并用荧光偏振法测量它们的膜流动性。发现网织红细胞与成熟红细胞的膜的剪切弹性模量(E)和表面粘度(μm)及膜的流动性有着明显的不同。这对研究由于贫血等原因造成的网织红细胞增多情况下,全血的微观流变特性有着重要的临床意义,对我们认识哺乳类网织红细胞的微观流变特性有重要理论意义及认识哺乳类动物网织红细胞向成熟红细胞转变的微观流变特性变化有着重要理论意义。According to Bishop method, a lot of reticulocytes were induced in mouse blood and were separated. Using a ektacytometry, the small deformation index and half time for deformation relaxation of the reticulcytes were measured. The value of membrane shear elastic modulus（E） and membrane viscosity （μm）were calculated through the formula with （ DI）d and t0.5 , Membrane fluidity was measured by fluorescence polarization method, It was found that membrane fluidity was increased, while membrane shear elastic modulus（E） and the membrane viscosity were decreased in the process of reticulocytes changing into erythrocytes. The experimental results are of both theoretical and clinical significance.

关 键 词：激光衍射法 剪切弹性模量(E) 表面粘度(μm) 膜的流动性 

分 类 号：R318[医药卫生—生物医学工程]