聚乳酸壳聚糖复合支架材料的生物相容性研究  

Biocompatibility of PLA/Chitosan Composite Scaffold Materials

作　　者：李立华[1] 焦延鹏[1] 李志忠[2] 丁珊[3] 邓德礼[2] 周长忍[1] 

机构地区：[1]暨南大学生命科技学院生物材料研究室,广州510632 [2]暨南大学第一附属医院骨科,广州510632 [3]暨南大学医药生物技术研究开发中心,广州510632

出　　处：《中国生物医学工程学报》2005年第4期503-506,共4页Chinese Journal of Biomedical Engineering

基　　金：国家"863"资助项目(2001AA625050);国家"973"资助项目(G199054306);广东省科技项目重大专项资助项目(A302020104)。

摘　　要：为改善聚乳酸作为骨组织工程支架材料降解速率过快、亲水性差和降解产物呈酸性等缺点,本研究制备了一系列高孔隙率的聚乳酸/壳聚糖三维多孔复合支架材料,通过软骨细胞培养、动物皮下和肌肉植入试验对其进行了生物相容性研究。软骨细胞培养试验表明软骨细胞能在复合支架材料贴附增殖,材料无明显毒性;植入试验结果显示纯聚乳酸在体内2个月左右已经降解吸收,失去力学强度,复合材料三个月后仍能保持一定的力学强度和形状,而且组织切片也同时表明复合材料的炎症反应远远低于纯聚乳酸材料。Polylactic acid（PLA） was a kind of popularly researched and used material in recent years except for several obvious weaknesses： biodegrading too fast, acidic degradation product, and hydrophobic. To solve aforementioned chemical defect, a series of PLA/chitosan composite materials were prepared by melting under vacuum. Chondrocyte cultivation,subcutaneous and intramuscular implantation were mainly discussed in this paper.The results showed that the cells could well adhere, grow and multiplicate on the surface of the materials. The plantation test revealed that the tissue response toward the implanted PLA/chitosan composite materials was mild, regardless of the ratio of PLA and chitosan. PLA materials were degraded and absorbed 2 months later in the body, while the composite materials could still keep certain strength and had far less inflammation.

关 键 词：聚乳酸 壳聚糖 骨组织工程 生物相容性 

分 类 号：R318[医药卫生—生物医学工程]