异黄酮活性成分对Aβ_(1-42)诱导的PC12细胞损伤的保护作用  

The protective effect of active components of isoflavones on the PC12 cells damage induced by Aβ_(1-42)

作　　者：杨红[1] 赵宇红[2] 陈伟强[1] 罗少洪[1] 金桂芳[1] 潘伟[1] 

机构地区：[1]广东药学院基础学院生物化学教研室,广东广州510224 [2]广东药学院药理学教研室,广东广州510224

出　　处：《中国药理学通报》2005年第9期1089-1091,共3页Chinese Pharmacological Bulletin

基　　金：广东省科技厅科技计划资助项目(No2004B30101001);广东省中医药局科研资助项目(No103079)

摘　　要：目的探讨异黄酮活性成分对β淀粉样蛋白(Aβ1-42)诱导的PC12细胞损伤的保护作用。方法从大豆中提取纯化异黄酮活性成分,以Aβ1-42诱导PC12细胞损伤为细胞模型,采用细胞形态学方法、MTT法、LDH法观察不同剂量的异黄酮活性成分对PC12细胞损伤的保护作用。结果异黄酮活性成分能有效地改善PC12细胞的形态,提高细胞存活率(P<0.05),保护组LDH活性低于损伤组(P<0.05)。结论大豆异黄酮活性成分对Aβ1-42诱导的PC12细胞损伤具有显著的保护作用。Aim To study the effect of nents of isoflavones on PC 12 cells damag active compoe induced with Aβ（1-42）. Methods The active components of isoflavones were extracted. Using PC12 cells damage induced by Aβ（1-42） as the model, the protective effect of active components was determined with morphological examination and MTF and LDH assays. Results The morphological change of the damaged PC12 cells wasimproved, and the survival rate of the cells was significantly increased （ P 〈 0. 05 ）. The LDH activity in the protective group was markedly decreased （P 〈 0.05）. Conclusion The protective effect of active components of isoflavones was significant on PC12 cells damage induced by Aβ（1-42）.

关 键 词：异黄酮活性成分 Β淀粉样蛋白 PC12细胞 

分 类 号：R282.71[医药卫生—中药学] R329.24[医药卫生—中医学]