骨形态生成蛋白在诱导骨形成过程中同时诱导血管内皮生长因子生成的剂量依赖性效应  

Dose-dependent effect of bone morphogenetic protein in inducing the generation of vascular endothelial growth factor during the procedure of inducing bone formation

作　　者：方秀统[1] 牛丰[1] 张新[1] 高鹏[1] 贾国伟[2] 丰景斌[3] 

机构地区：[1]吉林大学第一医院骨科,吉林省长春市130021 [2]通化铁路医院电诊科,吉林省通化市132001 [3]辽源市中医院骨科,吉林省辽源市134001

出　　处：《中国临床康复》2005年第30期73-75,i0002,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:血管生成与早期骨形成密切相关,而血管内皮生长因子是最有利的血管生成因子。探讨骨形态生成蛋白在骨生成过程中对血管内皮生长因子的影响。方法:实验于2004-05/08在吉林大学预防医学院地方病研究所完成。取12周龄雄性SD大鼠40只。随机分为4组,对照组、骨形态发生蛋白42mg组、骨形态发生蛋白44mg组、骨形态发生蛋白46mg组,每组10只。①模型建立:大鼠氯氨酮腹腔注射麻醉后,于右侧股骨前外侧长约2cm处分离至肌肉间隙,骨形态发生蛋白组置入充满不同剂量骨形态发生蛋白4的7mm明胶海绵,对照组肌间隙仅置入明胶海绵,缝合伤口。②肌肉组织标本病理观察:分别于术后3,7d每次每组随机选取5只在麻醉状态下处死,取肌肉组织标本进行光学显微镜观察。③肌肉组织血管内皮生长因子RNA的表达测定:确定大鼠血管内皮生长因子及内对照β-肌动蛋白引物序列,取聚合酶链反应扩增产物做琼脂糖凝胶电泳分析。结果:40只大鼠均进入结果分析。①各组大鼠肌肉组织标本病理改变:伤后3d骨形态发生蛋白各组比较差别不显著。伤后7d可见软骨细胞分泌的骨基质成分出现,并且随着骨形态发生蛋白4剂量的增加而增加。②各组大鼠肌肉组织标本血管内皮生长因子RNA的表达:伤后3d骨形态发生蛋白各组表达未检出。伤后7d电泳分析可见,血管内皮生长因子/β-肌动蛋白,骨形态发生蛋白42mg组,4mg组和6mg组均显著高于与对照组(0.92±0.14,1.07±0.11,1.09±0.19,0,P<0.05),骨形态发生蛋白46mg组显著高于骨形态发生蛋白2mg组(P<0.05)。结论:实验结果论证了骨形态发生蛋白引诱骨形成过程中刺激血管内皮生长因子的形成,并且随着骨形态发生蛋白4剂量的增加而增加,进一步促进和加速骨形成。AIM： The vascularization has close relation with bone formation earlier period, while vascular endothelial growth factor （VEGF） is the most advantageous vascularization factor. To explore the effect of bone morphogenetic protein on VEGF during the procedure of osteogenesis. METHODS： The experiment was completed in the Institute of Endemic Disease of Prophylactic Medicine College of Jilin University between May and August 2004. Forty male SD rats aged 12 weeks old were selected and divided into 4 groups by random, control group, 2 mg bone morphogenetic protein 4 group, 4 mg bone morphogenetic protein 4 group and 6 mg bone morphogenetie protein 4 group with 10 rats in each group.①The establishment of models： After the injection of chlorine ammonia keton through abdominal cavity and narcosis in rats, at about 2 cm anterior lateral of right-side femur separated till the interspace of muscle. The rats in bone morphogenetic protein group were injected with 7 mm gelatin sponge of bone morphogenetic protein 4 at different dosage. The rats in control group only injected with gelatin sponge in the interspace of muscle, and the cut was sutured.② Pathological observation of muscle tissue specimen： Five rats in every group were collected randomly and killed every time under drugged state post-operation 3, 7 days respectively. The muscle tissue specimen was gained to perform the observation of light microscope. ③ The evaluation of the expression of VEGF RNA in muscle tissue： The VEGF and internal controlled β-actin primer rank of rats were defined. The amplification product of polymerase chain reaction （PCR） was gathered and performed agarose gel electrophoresis analysis. RESULTS： Totally 40 rats were involved in the result analysis. ①The pathological change of muscle tissue specimen of rats in every group： The difference was insignificant in every bone morphogenetic protein group 3 days after injury. The bone matrix component excreted by chondrocyte emerged 7 days after injury, and increased as

关 键 词：骨折愈合 受体 血管内皮生成因子 骨形态发生蛋白质类 

分 类 号：R68[医药卫生—骨科学]