酸性成纤维细胞生长因子表达对急性放射性皮肤溃疡创面修复的影响  

Influence of the expression of acidic fibroblast growth factor on the healing of acute radiation-induced skin ulcers

作　　者：王红光 曹卫红[2] 邹晓防[2] 杨志祥[3] 谷庆阳[3] 王德文[3] 

机构地区：[1]解放军92805部队医院感染科 [2]解放军总医院三○四临床部烧伤科 [3]解放军军事医学科学院放射医学研究所

出　　处：《中国临床康复》2005年第30期107-109,i0003,共4页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨急性放射性皮肤溃疡形成早期酸性成纤维细胞生长因子及其受体的表达和意义。方法:实验于1999-06/2004-12在军事医学科学院完成。选取二级健康雌性Wistar大鼠80只,随机分为照射组40只、创伤组30只、正常对照组10只。照射组以60Coγ射线50Gy单次局部照射建立急性放射性皮肤溃疡动物模型,照射部位为双后大腿、臀部及全尾;创伤组于背部制作直径1.5cm单纯皮肤伤口动物模型;正常对照组未作任何处理。采用免疫组化、原位杂交等方法检测创面内酸性成纤维细胞生长因子及其受体的表达。400倍条件下光镜观察其相对含量,以视野内阳性细胞数量为准,分为阴性(视野内无阳性信号)、弱阳性(视野内可见10个以下阳性信号)、阳性(视野内可见11～30个阳性信号)、强阳性(视野内可见30个以上阳性信号)。结果:实验共纳入80只大鼠,进入结果分析73只,死亡7只。①照射组与创伤组大体观察:照射组照射后1～7d未出现异常反应,第9天出现尾根部红肿及足底轻度红肿,第28天尾根部与大腿内侧脱毛、糜烂及浅小溃疡达高峰,第35天伤口仍未愈合,溃疡周围可见新生上皮迟缓长入,第55天溃疡周围新生上皮部分覆盖创面,伤口有收缩趋势,但仍未愈合。创伤组皮肤伤口均经历炎症渗出、伤口收缩、瘢痕愈合阶段,伤后1～2d炎性渗出较多,第3天伤口开始收缩,伤后18d时伤口全部愈合。②照射组与创伤组基本病理学变化:照射组:溃疡前期表皮细胞及毛囊上皮肿胀、核固缩、碎裂,真皮及皮下组织出现充血性血管反应,胶原纤维肿胀、融解、断裂、水肿、排列紊乱,并有灶状表皮浅层缺失灶;溃疡期溃疡表面为坏死组织层,其下为增生不良的肉芽组织,其内极少见新生血管,胶原纤维变性、融解、断裂、水肿,可见成纤维细胞松散聚集形成的细胞团。创伤组:伤后1～3d,创面大量炎细胞、浆AIM： To investigate the expressions and significances of acidic fibroblast growth factor （a-FGF） and its receptor at the early stage of acute radiationinduced skin ulcers. METHODS： The experiment was finished in the Academy of Military Medical Sciences from June 1999 to December 2004. Eighty healthy second-degree female Wistar rats were enrolled in the study, .and randomized into three groups： radiation group （n=40）, trauma group （n=30） and normal control group （n=10）. In the radiation group, tat models of acute radiation-induced skin injury were made with 50 Gy ^60Coγ-rays on two lower limbs, buttock and tails. In the trauma group, models of skin wound at the back with the diameter of 1.5 cm were made. Rats in the normal control group did not receive any treatment. Immunohistochemieal staining and in situ hybridization were used to detect the expressions of a-FGF and its receptor in simple skin wounds and acute radiation-induced skin ulcers. The relative content was observed under light microscope, and divided into four degrees according to the number of positive cells： negative （none positive signals in sights）, weak positive （less than 10 positive signals in sights）, positive （10-30 positive signals in sights）, strong positive （more than 30 positive signals in sights）. RESULTS： Eighty rats were enrolled in the study, 7 died and 73 were involved in the analysis of results. ① Gross observation： In the radiation group, no abnormal reaction was shown from 1 to 7 days, red swelling appeared at 9 days on tails and sole of feet, hair abscission, erosion, small ulcers increased maximally at 28 days on tails and inside of lower limbs, neonate epithelia grow slowly at 35 days, the ulcers contracted and partially healed at 55 days; In the trauma group, the wounds healing stages included inflammation exudation,wound contraction, scar healing stages, inflammation exudation was rich from 1 to 2 days,wound contraction appeared at 3 days, and all wounds healed at 18 days after in

关 键 词：受体 成纤维细胞生长因子 辐射损伤 急性病 内皮生长因子 皮肤溃疡 伤口愈合 疾病模型 动物 

分 类 号：R751[医药卫生—皮肤病学与性病学]