自制复方中药对膝骨性关节炎家兔血液流变及病理形态的影响  

Effects of self-made compound traditional Chinese medicine on hemorheology and pathomorphology in rabbits with knee osteoarthritis

作　　者：陈述祥[1] 司徒坚[1] 杨运东[1] 赵崇达[1] 丁林坚[1] 刘红光[1] 

机构地区：[1]江门市五邑中医院骨科(暨南大学医学院第六附属医院),广东省江门市529031

出　　处：《中国临床康复》2005年第30期147-149,共3页Chinese Journal of Clinical Rehabilitation

基　　金：江门市科技局立项资助课题(20030302)~~

摘　　要：目的:观察院内自制复方中药制剂骨痹灵口服液对家兔膝关节骨性关节炎模型在血液流变及病理形态方面的影响,并与芬必得作比较。方法:实验于2004-01/08在第一军医大学珠江医院完成,选用健康新西兰家兔36只,随机分为3组,每组12只。空白对照组,芬必得组,骨痹灵口服液组(每mL口服液含生药约1g,成份为鸡血藤15g、黄芪15g、骨碎补15g、肉苁蓉10g、防风10g、鹿角胶10g、怀牛膝10g、当归10g、白芍10g)。在造模前抽取空白对照组家兔耳缘静脉血,测定血液流变学各指标(设为空白对照)。取家兔左膝伸直位管型石膏固定8周的方法建立骨关节炎动物模型。空白对照组以生理盐水15mL灌胃,2次/d;芬必得组以芬必得胶囊15mg灌胃,2次/d;骨痹灵口服液组以骨痹灵口服液15mL灌胃,2次/d。各组给药从造模1周开始,连续4周后,抽血测定血液流变学的各项指标。5周后,进行滑膜、软骨标本的组织学观察,并按GoldenbergandCohen标准评分评估各组动物滑膜炎症程度。结果:36只家兔没有出现厌食或其他消化系统症状,没有出现体质量下降及死亡,所有家兔均纳入指标检测。①血液流变学指标测定结果:灌胃4周后,空白对照组血小板聚集率、血浆黏度、全血黏度低切变率(3/s)明显高于灌胃前[(90.92±5.02)%,(73.54±4.25)%;(4.22±4.32),(1.73±1.34)mPa·s;(10.12±1.50),(8.05±3.15)mPa·s,P<0.01]。骨痹灵口服液组血小板聚集率、血浆黏度、全血黏度低切(3/s)均显著低于空白对照组[(81.50±3.22)%,(90.92±5.02)%;(2.25±4.86),(4.22±4.32)mPa·s;(8.66±2.30),(10.12±1.50)mPa·s,P<0.01]。而芬必得组仅在血小板聚集率上显著低于空白对照组[(78.30±1.66)%,(90.92±5.02)%,P<0.01]。骨痹灵口服液组血浆黏度、全血黏度低切(3/s)低于芬必得组[(2.25±4.86),(3.96±1.05)mPa·s;(8.66±2.30),(9.80±2.36)mPa·s,P<0.01]。②组织病理学观察结果:大体标本观察:各组造模侧膝关节5周后仍�AIM：To observe the effects of Gubiling oral solution, a self-made compound traditional Chinese medicine, on the hemorheology and pathomorphology in rabbits with knee osteoarthritis, and compare with Fenbid （ibuprofen sustained-release）. METHODS：The experiment was carried out in the Zhujiang Hospital of the First Affiliated Hospital of Chinese between January and August 2004. Thirty-six healthy New Zealand rabbits were randomly divided into 3 groups with 12 rabbits in each group： blank control group, Fenbid group and Gubiling oral solution group. The Gubiling oral solution consisted of 15 g subereet spatholobus stem, 15 g membranous milkvetch root, 15 g Fortume＇ s drynaria rhizome, 10 g desertliving cistanche, 10 g divaricate saposhnikovia root, 10 g deerhorn glue, 10 g twotooth achyranthes root, 10 g Chinese angelica and 10 g white peony root, 1 mL oral solution con-tained about 1 g crude drug. Before model establishment, venous blood samples were drawn from the ear edge of rabbits in the blank control group to determine the hemorrheologic indexes （blank control group）. Models of osteoarthritis were established by means of fixation of left knee with plas-ter cast at straight position for 8 weeks. Rabbits in the blank control group, Fenbid group and Gubiling oral solution group were treated with gastric perfusions of saline （15 mL）, Fenbid capsule （15 mg） and Gubiling oral solution （15 mL） respectively, twice a day for 4 weeks. Blood samples were drawn to determine the hemorrheologic indexes at the first week of model establishment and after 4 continuous weeks. After 5 weeks, the histological observation of synovium and cartilage samples was performed, and the severity of synovial inflammation was evaluated with Goldenberg and Cohen standard scores. RESULTS： No anorexia or other symptoms of digestive system were observed, and no decrease of body mass and death occurred, all the 36 rabbits were involved. ① The results of the determination of hemorrheologic indexes： Four weeks after

关 键 词：骨关节炎 动物实验 中药疗法 血液流变学 病理学 

分 类 号：R684[医药卫生—骨科学]