刺五加多糖的提取及其抗氧化性  

Extraction of Acanthopanacis Senticosi Polysaccharides and Their Antioxidative Effect

作　　者：孟庆繁[1] 于笑坤[1] 徐睦芸[1] 李明亮[1] 高智慧[1] 范豪[1] 初宇卓[1] 滕利荣[1] 

机构地区：[1]吉林大学生命科学学院,长春130012

出　　处：《吉林大学学报（理学版）》2005年第5期683-686,共4页Journal of Jilin University:Science Edition

基　　金：全国教育科学"十五"规划教育部重点项目基金(批准号:DIA030165)

摘　　要：用热水浸提法提取刺五加多糖,用乙醇对其分级沉淀,当乙醇的体积为提取液的1倍时,多糖沉淀量最大,随着乙醇用量增加,各级产物减少,其多糖的总收率为原刺五加质量的0.54%.用邻苯三酚法及fenton体系测定各分级产物对O2-和.OH的抑制作用,随着乙醇用量增加,其产物的抗氧化能力增强,当乙醇用量是提取液体积的6倍时,其产物对O2-的清除率最大可达47.7%;当乙醇用量达4倍时,其产物对.OH的清除率最大达68.2%.刺五加多糖对红细胞膜脂质过氧化的抑制作用随糖浓度的增加而加大,当多糖浓度在0.4 g/L以上时,不仅H2O2对红细胞膜的氧化作用完全被抑制,而且对红细胞膜产生还原作用.Acanthopanacis Senticosi Polysaccharides （ASPs） were extracted by hot water. ASPs in the filtrate were successively precipitated with alcohol. When the quantity of alcohol was equal to the volume of the ASPs filtrate, the production yield of ASPs was the highest. The yields of the fraction productions were decreasing with the increase of the quantitiy of alcohol and the total yield of ASPs was up to 0.54%. The inhibitions of the fraction productions to O2^- and · OH were assayed by the method of pyrogallol in the fenton system. The results show that the antioxidative effect of the fraction productions is improved with the increase of the quantity of alcohol. When the quantity of alcohol was 6 times the volume of ASPs filtrate, the elimination rate of the production to O2^- could reach to the highest value of 47.7%. When the quantity of alcohol was 4 times the volume of ASPs filtrate, the elimination rate of the production to · OH could reach to the highest value of 68.2%. The inhibition of ASPs to the lipid peroxidation of the erythrocyte membrane was improved with the increase of the concentration of ASPs. When the concentration of ASPs was above 0.4 g/L, ASPs could not only inhibit the lipid peroxidation but also produce some reductive effect to the erythrocyte membrane.

关 键 词：刺五加多糖 提取 生物活性 

分 类 号：Q539[生物学—生物化学]