血型单克隆抗体试剂稳定剂的研究  

Study on stabilizers of anti-A and anti-B blood grouping reagents (monoclonal antibody)

作　　者：杨福军[1] 罗广[1] 彭林峰[1] 马超[1] 张正雷[1] 张甲菊[1] 张兰香[1] 

机构地区：[1]兰州生物制品研究所,兰州730046

出　　处：《微生物学免疫学进展》2005年第3期37-40,共4页Progress In Microbiology and Immunology

摘　　要：为了进一步提高抗A、抗B血型单克隆抗体试剂的稳定性,实验中分别以不同配比的甘油、蔗糖、明胶和EDTA为稳定剂主要成分加入血型单抗试剂,放置在不同条件下观察试剂的稳定性.结果表明,所筛选出的一种稳定剂对血型单抗试剂具有良好的保护作用,试剂在2～8℃条件下放置12个月,凝集效价保持不变,并且该稳定剂对血型单抗的特异性、亲和力、凝集效价和凝集强度均无影响.To improve the stability of anti-A and anti-B blood grouping reagents （ monoclonal antibody）, glycerin, sucrose, gelatin and EDTA were used as components of stabilizers in the designed formulations with different proportion. The results showed that the selected formulation had a significant protective effect on blood grouping reagents, and the agglutination titern of reagents were kept immovability at 2 - 8℃ condition for 12 months. The results also showed that the selected stabilizers had no effect on reagent＇s specificity, affinity, agglutination titer and agglutination intensity.

关 键 词：血型试剂 单克隆抗体 稳定剂 

分 类 号：R446.6[医药卫生—诊断学]