ALD-DNA对巨噬细胞株RAW264.7的刺激作用  

Immunostimulatory Effect of ALD-DNA on Murine Macrophage Cell Line RAW264.7 and Its Significance

作　　者：乔滨[1] 徐薇[1] 陈刚[1] 张凤[1] 杨秀利[1] 熊思东[1] 

机构地区：[1]复旦大学上海医学院免疫学系-教育部分子医学重点实验室-上海基因与疫苗研究中心,上海200032

出　　处：《复旦学报（医学版）》2005年第5期505-508,共4页Fudan University Journal of Medical Sciences

基　　金：上海市科委优秀学科带头人计划(04XD14003);国家自然科学基金资助项目(30200259)

摘　　要：目的体外观察ConA活化淋巴细胞来源的DNA(ALDDNA)对BALB/c小鼠巨噬细胞株RAW264.7的刺激作用。方法将ALDDNA及未活化淋巴细胞来源的DNA(UnALDDNA)分别与BALB/c小鼠来源的巨噬细胞株RAW264.7孵育48h,然后以四甲基偶氮唑蓝(MTT)法检测细胞的增殖情况,流式细胞仪测定细胞表面分子的表达,ELISA检测白细胞介素6(IL6)、白细胞介素12(IL12)和肿瘤坏死因子α(TNFα)的分泌情况。结果ALDDNA在体外能够明显刺激细胞发生增殖,上调细胞表面主要组织相容性复合体(MHC)II类分子以及共刺激分子CD40、CD80和CD86的表达,并能够诱导细胞分泌IL6、IL12和TNFα。结论ALDDNA对RAW264.7细胞具有免疫刺激作用。Purpose To observe the immunostimulatory effect of Con A-activated lymphocytes derived DNA（ALD-DNA） on RAW264.7 in vitro. Methods Macrophage cell line RAW264.7 from BALB/c mice was incubated 48 hours with ALD-DNA and unactivated lymphocytes derived DNA （UnALD-DNA）, respectively. The proliferation of RAW264.7 was determined by MTT colorimetric assay. The cell-surface expression of MHC class Ⅱ and costimulatory molecules CD40, CD80, CD86 was analyzed by FACS. The production of IL-6, IL-12 and TNF-α by RAW264.7 was assessed by ELISA. Results ALD-DNA could trigger proliferation of RAW264. 7, up-regulate MHC class Ⅱ and costimulatory molecules prensent on RAW264.7 in vitro,and stimulate secretion of IL-6, IL-12 and TNF-α. Conclusions ALD-DNA has the immunostimulatory effect on RAW264.7.

关 键 词：活化淋巴细胞来源的DNA 巨噬细胞 免疫刺激 

分 类 号：R392.11[医药卫生—免疫学]