浙江省某农村地区猪粪便与胆汁中戊型肝炎病毒的检测和部分序列分析  

Detection and Analysis of Partial Sequences Isolated from Swine in Rural Area of Zhejiang Province

作　　者：张潇[1] 郑英杰[1] 王法弟[2] 高眉扬[1] 朱建福[2] 姜庆五[1] 

机构地区：[1]复旦大学公共卫生学院流行病学教研室-公共卫生安全教育部重点实验室,上海200032 [2]浙江省德清县疾病预防控制中心,313200

出　　处：《复旦学报（医学版）》2005年第5期586-589,共4页Fudan University Journal of Medical Sciences

基　　金：复旦大学青年科学基金资助项目

摘　　要：目的调查浙江省某农村地区猪群中戊型肝炎病毒的感染状况,探讨猪戊型肝炎病毒与人戊型肝炎病毒的关系。方法应用逆转录-巢式聚合酶链法(RTnPCR)对当地某养猪场的猪粪便标本和生猪屠宰中心的猪胆汁标本进行HEVRNA的检测,并对部分阳性标本进行克隆测序和序列分析。结果132份猪粪便标本中13份为HEVRNA阳性,阳性率为9.85%;160份猪胆汁标本中有11份为阳性,阳性率为6.88%。序列分析发现猪HEV相互之间在ORF2部分区域(nt6317～6466)的核苷酸序列的同源性为90.0%～100.0%,与当地人株核苷酸序列的同源性为88.7%～100.0%,与Ⅰ、Ⅱ、Ⅲ和Ⅳ型的同源性分别为78.7%～84.0%,80.0%～85.3%,76.0%～83.3%和84.7%～95.3%。结论该地区猪HEV与当地人HEV同属HEVⅣ型。Purpose To investigate the HEV infection in swine and the genotype relationship between swine HEV and human HEV. Methods HEV RNA was detected using RT-nPCR with ORF2 primers. The positive PCR products were cloned and sequenced. Results Totally 13 of 132（9.85 % ） pigs＇ stool samples, 11 of 160 pigs＇ bile samples were positive for HEV RNA. The sequence analysis showed that the identiy at nucleotide level was 90.0%--100.0% among them. They had 88.7%--100.0% homology at the nucleotide level with the HEV isolated from local people. They shared 78.7 %--84.0 % , 80.0 %--85. 3 %, 76.0 %--83.3 % and 84.7 %--95.3 % nucleotide sequence identity with HEV genotype Ⅰ, Ⅱ , Ⅲ and Ⅳ ,respectively,in the region（nt6317--6466）. Conclusions The hepatitis E virus circulated in human and swine in the area belonged to genotype Ⅳ.

关 键 词：戊型肝炎病毒 基因型 人兽共患性疾病 

分 类 号：R512.6[医药卫生—内科学]