两种DNA探针在肺炎链球菌DNA检测中的应用价值  

Evaluation of applicability for two types of DNA probes in detection of Streptococcus pneumoniae

作　　者：樊慧珍[1] 于化鹏[1] 黄文杰[2] 唐银丽[1] 

机构地区：[1]南方医科大学附属珠江医院呼吸科,广州510280 [2]广州军区广州总医院呼吸科,广州510010

出　　处：《临床检验杂志》2005年第5期344-346,共3页Chinese Journal of Clinical Laboratory Science

基　　金：广东省社会发展攻关基金(2002C30405)。

摘　　要：目的研究寡核苷酸探针和长链DNA探针在肺炎链球菌检测中的诊断价值。方法合成肺炎链球菌特异的21bp寡核苷酸探针和263bp长链DNA探针并用生物素标记,两种探针分别与细菌全染色体DNA进行斑点杂交,比较两种探针检测痰液标本的敏感性和特异性,并与痰培养法比较。结果寡核苷酸探针和263bp探针检测肺炎链球菌基因组DNA的敏感性分别是100ng和1ng。培养法和杂交法同时检测100份痰标本,培养法、21bp探针和263bp探针的阳性检出率分别是7%、6%和17%。结论263bp长链DNA探针是检测肺炎链球菌DNA的较佳选择。Objective To study the diagnostic value of oligonucleotide probe and long-chain DNA probe in detection of Streptococcus pneumoniae （SP）. Methods The 21 bp oligonucleotide probe and 263 bp DNA probe of streptococcus pneumoniae were synthesized and labeled with biotin. The two types of probes were hybridized with genomic DNA of streptococcus pneumoniae respectively to compare their sensitivity and specificity of the detection. Clinical sputum samples were also detected by the 2 types of probe simultaneously. Results Both the two probes exhibited high specificity. The sensitivities of oligonucleotide probe and 263 bp DNA probe were 100ng and lng of DNA respectively in the detection for SP DNA. The positive rates of culture, hybridization by 21 bp probe and 263 bp probe were 7%, 6% and 17% respectively in the detection of 100 sputum samples. Coneltmions The 263 bp DNA probe is the optimal choice for the detection of streptococcus pneumoniae by dot-blot hybridization.

关 键 词：探针 肺炎链球菌 杂交 

分 类 号：R446.5[医药卫生—诊断学]