反相高效液相色谱法测定人血浆中氯硝西泮浓度  

RP-HPLC determination of clonazepam in human plasma by solid-phase extraction

作　　者：马武翔 田国强 陈华良 

机构地区：[1]绍兴市第七人民医院药剂科,绍兴312000

出　　处：《中国临床药学杂志》2005年第5期305-307,共3页Chinese Journal of Clinical Pharmacy

基　　金：绍兴市科技计划项目(编号20021110)

摘　　要：目的采用反相高效液相色谱法测定人血浆中氯硝西泮的浓度.方法血浆经碱化后,用OASIS固相萃取小柱提取血浆中氯硝西泮,采用HPLC法紫外检测器检测.色谱柱为Shim-Pack C18柱(150 mm×4.6 mm,5 μm),保护柱为Shim-Pack C1s柱,流动相为水-乙腈(65:35,V/V),流速为1.0 mL·min-1,检测波长252 nm.结果测定方法在氯硝西泮浓度为5～100μg·L-1(r=0.999 3)范围内具良好线性关系,萃取回收率在91.9%～98.2%之间.日内、日间RSD在3.7%～1.3%之间,最低检测浓度为2μg·L-1.结论本测定方法适用于临床氯硝西泮浓度检测的要求.AIM To develop a simple, sensitive and rapid RP-HPLC with ultraviolet detector method for the determination of clonazepam in human plasma. METHODS The clozapine in plasma samples（1mL） was processed by Waters OASIS solid-phase extractor, the separation was made by using a reversed-phase Shim-Pack C18 column with isocratic mobile phase consisting of water-acetonitrile （65：35, V/V） ;the flow rate was 1.0 mL· min^-1 and the detective wavelenth was 252 nm. RESULTS A linearity was obtained in the range of 5 - 100 μg· L^-1 （ r = 0. 999 3）, the lowest detectable concentration in plasma was 2 μg· L^-1. The extraction recovery was between 91.9% -98.2%. The relative standard deviations of intra-day and inter-day assay were within 3.7 % - 11.3 %. CONCLUSION The established method is showen to be sensitive, accurate arid simple for the determination of clonazepam levels in human plasma. It is suitable for the pharmacokinetic and TDM study of clonazepam.

关 键 词：固相萃取 高效液相色谱法 血药浓度 氯硝西泮 

分 类 号：R96[医药卫生—药理学]