银杏叶提取物对酒精所致大鼠睾丸氧化损伤的保护作用的研究  

Protective effects of extract of ginkgo biloba against ethanol-induced oxidative injury in rat testes

作　　者：李珂[1] 姚平[1] 周绍良[1] 宋方方[1] 章锡平[1] 孙秀发[1] 刘烈刚[1] 

机构地区：[1]华中科技大学同济医学院公共卫生学院营养与食品卫生学系,武汉430030

出　　处：《卫生研究》2005年第5期559-562,共4页Journal of Hygiene Research

基　　金：国家自然科学基金项目(No.30271130)

摘　　要：目的研究银杏叶提取物(EGB)对酒精所致大鼠睾丸氧化损伤的预防保护作用。方法雄性SD大鼠30%酒精灌胃(2.37g/kgbw)前,EGB采用分组(低剂量组48μg/gbw和高剂量组96μg/gbw)预防性给药的方法。于实验90d检测大鼠睾丸组织中超氧化物歧化酶(SOD)、谷胱甘肽转移酶(GST)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、和谷胱甘肽(GSH)、活性氧(ROS)以及丙二醛(MDA)的含量。通过亚细胞分离方法提取大鼠睾丸微粒体,测定微粒体血红素氧化酶-1(HO-1)活性。采用RT-PCR检测睾丸组织中血红素氧化酶-1(HO-1)mRNA表达水平。结果慢性酒精摄入90d后,EGB组睾丸匀浆ROS、MDA较酒精组有明显降低(P<0.05);相反,GST、G-Px、CAT、SOD、HO-1活性以及GSH均有显著性升高(P<0.05);EGB诱导HO-1高表达。结论长期慢性酒精摄入引起大鼠睾丸氧化损伤。EGB可作为一种预防性的抗氧化剂减轻这种氧化损伤;其机制可能与诱导HO-1高表达,清除自由基,抑制脂质过氧化反应有关。Objective This study was designed to investigate whether EGB protect against ethanol-induced oxidative injury in rat testes. Methods The rats were pretreated with EGB （4.8,9.6mg/100g bw per day） before 30% ethanol administration （2.37g/kg bw by gastric incubation）. Ninety days later, the rats were killed in order to measure the activities of Superoxide Dismutase （ SOD ）, Glutathione Reductase （ GST）, Glutathione Peroxidase （ GSH-Px ）, Catalase （CAT） and the contents of Glutathione（GSH）, Reactive Oxygen Species（ROS）, Malondialdehyde（ MDA）in rat testes. The microsomal fractions were obtained by the method of differential centrifugation, and HO-1 （Heme Oxygenase-1 ） activity in testicular microsomal was measured. To examine the expression of HO-1 mRNA by reverse transcription polymerase chain reaction （RT-PCR）. Results Compared with ethanol group, ROS, and MDA contents significantly decreased in EGB group testes, respectively（P 〈 0.05）. GST, GSH-Px, CAT, SOD activities, and GSH content in EGB group testes elevated markedly（ P 〈 0.05）.EGB could enhance HO-lmRNA expression remarkably. Conclusion It is suggested that EGB as a preventive antioxidant can protect against ethanol-induced testicular injury,which may be associated with HO-1 activity enhancement, free radicals elimination, lipid per-oxidation reaction inhibition.

关 键 词：银杏叶提取物 氧化损伤 抗氧化酶 血红素氧化酶-1 

分 类 号：R961[医药卫生—药理学] Q579.1[医药卫生—药学]