茶树内生防病和农药降解菌的分离  

Isolation of Endophytic Antifungal and Pesticide Degrading Bacteria from Tea Plant

作　　者：洪永聪[1] 辛伟 来玉宾[1] 翁昕[1] 胡方平[1] 

机构地区：[1]福建农林大学农药与化学生物学教育部重点实验室,福建福州350002 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《茶叶科学》2005年第3期183-188,共6页Journal of Tea Science

基　　金：国家自然科学基金资助项目(No.30370968)

摘　　要：对不同茶树品种的健叶和病叶上分离的内生细菌进行了筛选和鉴定.结果表明,茶树体内存在大量的内生细菌,各品种间内生细菌的数量为2.9×106～39.4×106 cfu/(g·fw).内生细菌的生物功能测定结果表明,菌株TL2的拮抗能力强,先接种菌株TL2 24h后再接种茶轮斑病菌的防病效果好;同时菌株TL2对氯氰菊酯也表现出较强的降解能力;另外菌株TL2能在茶树上内生定殖.经鉴定,菌株TL2为枯草芽孢杆菌(Bacillus subtilis).The screening and identification of endophytic bacteria from the healthy and diseased leaves of various tea cultivar plants were conducted. Results showed that the endophytic bacteria were accounted in 2.9×10^6-39.4×10^6 cfu/（g·fw） in tea cultivars plants. The TL2 stain showed strongly antagonistic activity against leaf fungal pathogens of tea-plant. It showed a good protecting effect when inoculated the TL2 strain bacteria firstly than inoculated the tea grey blight pathogen（Pestallozzia theae） later, Besides, it showed a strong degrative activity to cypermethrin. The TL2 bacteria strain could colonize within tea plant. According to the results of identification, the TL2 bacteria strain was identified as bacillas subtilis.

关 键 词：茶树 内生细菌 防病 农药降解 枯草芽孢杆菌 

分 类 号：S182[农业科学—农业基础科学]