复方血竭胶囊对大鼠局灶性脑缺血再灌注损伤的拮抗作用  

Protective Effects of Compound Xuejie Capsule on Focal Cerebral Ischemia-Reperfusion Injury in Rats

作　　者：虞冬辉[1] 汪晖[1] 吴基良[2] 余薇[2] 

机构地区：[1]武汉大学医学院药理学系 [2]咸宁学院药学院药理学系

出　　处：《咸宁学院学报（医学版）》2005年第5期379-381,共3页Journal of Xianning Univarsity(medical Sciences)

摘　　要：目的探讨复方血竭胶囊(CXJC)对局灶性脑缺血再灌注损伤大鼠的作用。方法雄性SD大鼠40只,随机分为假手术组、缺血再灌注对照组、CXJC低剂量组(0.14 g.kg-1)、中剂量组(0.56 g.kg-1)和高剂量组(1.12 g.kg-1),每组8只。采用大鼠大脑中动脉栓塞法制备大鼠脑缺血再灌注模型。缺血2 h再灌注24 h后,采用5级评分法判定大鼠神经功能缺损,按干湿重法计算脑组织含水量,TTC染色观察梗死灶体积。结果低、中、高剂量CXJC均能降低缺血2 h再灌注3 h时的神经症状评分,其评分与模型对照组相比有显著性差异(P<0.01),其中中剂量组于缺血2 h再灌6 h及24 h评分仍低于模型组,其差异有显著性(P<0.05)。低、中、高剂量CXJC组均能降低脑水肿和脑梗死体积,与模型对照组比较有显著性差异(P<0.01或P<0.05)。结论CXJC对脑缺血再灌注损伤具有保护作用。Objective To explore the effects of Compound Xuejie Capsule （CXJC） on focal cerebral ischemia-reperfusion injury in rats. Methods 40 male Sprague-Dawley rats were randomly divided into five groups： sham group, ischemia-reperfusion group, CXJC groups of low dose （0. 14 g ·kg^-1）, middle dose （0. 56 g·kg^-1） and high dose （1.12 g ·kg^-1）. There were 8 animals in each group. The models of middle cerebral artery occlusion （MCAO） were established in rats. At 24h after MCAO, the extent of neurological deficit by scores grading on a scale of 0-4, cerebral edema with wet/dry weighing recorded and the size of cerebral infarction by TTC staining technique were determined. Results CXJC 0. 14, 0. 56 and 1.12 g·kg^-1 were found decreasing neurological deficit scores at 3h after ischemia-reperfusion （P〈0. 01）, the scores at 6h and 24h in CXJC 0. 56 g·kg^-1 group was still lower than that of the ischemia-reperfusion group （P〈0. 05）. CXJC of three doses also decreased both infarction volumes and cerebral water contents （P〈0.01 or cerebral ischemie-reperfusion injury in rats. P〈0. 05）. Conclusion CXJC has protective effects on cerebral ischemie-reperfusion injury in rats.

关 键 词：复方血竭胶囊 脑缺血再灌注 

分 类 号：R285.5[医药卫生—中药学]