水-有机溶剂混合体系中辣根过氧化物酶的催化反应  

Studies on horseradish peroxidase in water - organic solvents

作　　者：陈建波[1] 夏春谷[2] 李树本[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室,兰州730000

出　　处：《上海师范大学学报（自然科学版）》2005年第3期71-75,共5页Journal of Shanghai Normal University(Natural Sciences)

基　　金：上海师范大学青年基金(DKL404).

摘　　要：运用分光光度法和傅立叶变换红外光谱研究了辣根过氧化物酶(HRP)在甲醇、乙腈和二氧六环与水互溶溶剂体系中的性质变化,研究结果显示不同种类和含量的有机溶剂都使HRP有不同程度的失活,HRP在有机溶剂中的米氏常数(Km)增大了,但HRP的活性点结构没有遭到破坏,只是将活性部位从一个包埋于酶分子内部的、疏水的环境暴露于极性的有机溶剂中.傅立叶变换红外光谱研究结果显示在有机溶剂中酶分子的二级结构发生了变化,推测可能是酶在有机溶剂中的活性降低的原因.The properties of Horseradish peroxidase （HRP） were investigated in water - miscible organic solvents including methanol, acetonitrile and dioxane by using UV - Vis spectrophotometer and Fourier Transform Infrared （ FTIR）. Results indicated that enzyme denaturation occurred in different volumes of. water-miscible organic solvents and the Km values of HRP in water-organic solvents increased. The absorbance spectra of HRP in water-organic solvents showed the same major Soret absorpion as in aqueous solution, though the intensity of the Soret absorption increased. FTIR analysis indicated that the secondary structure of enzyme was affected by organic solvents greatly. It was proposed that the abovementioned effect may account for the decrease of enzyme＇s activity in water-organic solvents.

关 键 词：有机溶剂 辣根过氧化物酶 傅立叶变换红外光谱 

分 类 号：O643.32[理学—物理化学]