睾丸局部感染时Leydig细胞IL-1、IL-6、TGF-β和Fas、FasL转录水平的变化  

Changes of IL-1,IL-6,TGF-β,Fas and FasL expressed by Leydig cell in testis infection

作　　者：张颖德[1] 席晔斌[1] 李伟毅[1] 陈广洁[1] 王保国[1] 沈天伟[1] 包士三[2] 

机构地区：[1]上海第二医科大学上海市免疫学研究所,上海200025 [2]悉尼大学病理系

出　　处：《中国免疫学杂志》2005年第9期678-681,686,共5页Chinese Journal of Immunology

基　　金：上海市高等学校科学技术发展基金(02BK08)资助项目

摘　　要：目的:研究Leydig细胞在抗感染中的免疫调节作用。方法:以溶脲脲原体(UU)直接注入大鼠膀胱拟上行性感染的途径,制备大鼠睾丸感染的动物模型,分别在UU感染的1周、2周、3周处死大鼠,分离取得睾丸组织,观察组织的病理变化,并分离获得高纯度的Leydig细胞,抽提总RNA,用RTPCR方法比较UU感染组与正常对照组之间IL1、IL6、TGFβ和Fas、FasLmRNA表达的差异。结果:与正常对照组相比,UU感染的大鼠睾丸组织发生明显的病理改变,IL1、IL6、TGFβ均升高,Fas表达下降、FasL表达升高。结论:大鼠Leydig细胞在抗感染免疫中,可通过改变IL1、IL6、TGFβ和Fas、FasL转录表达格局来发挥抗UU的免疫调节作用。Objective： To study the effect of immune regulation of the rat Leydig cens in testis infection. Methods： UU was injected into the bladders of rats, which mimics the ascending infection pathway, while using culture medium injection as control. After 1,2,3 w respectively, the rats were sacrificed to observe the pathological alterations in testis by histological examination. Leydig cell were also separated from testis, the comparation of levels of IL-1,IL-6,TGF-β, Fas and FasL mRNA expression among control and UU infected groups was made by RT-PCR. Results： Compared with control, in UU infected group, the levels of IL-1, IL-6, TGF-β increased, the level of Fas decreased, the level of FasL increased. Conclusion： Leydig cell can play the role of immtme regulation, with the expressing changes of IL-1, IL-6,TGF-β, Fas and FasL in the infection of testis in rat.

关 键 词：Leyais细胞 免疫调节 IL-1 IL-6 TGF-Β FAS FASL 

分 类 号：R392.12[医药卫生—免疫学] R392.23[医药卫生—基础医学]