黄芪抗MNU致大鼠视网膜损伤的作用  

Protection of Astragalus against MNU-induced retinal damage in SD rats

作　　者：杨锦南[1] 何瑞芳[2] 陈金卯[3] 林少春[3] 李岱[3] 胡世兴[3] 

机构地区：[1]新乡医学院药学院,河南新乡453003 [2]新乡医学院生理室,河南新乡453003 [3]中山大学中山眼科中心,广东广州510060

出　　处：《中成药》2005年第9期1065-1067,共3页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(30300467);河南省科技攻关项目(0523030700)

摘　　要：目的观察黄芪注射液对N甲基N亚硝脲(MNU)诱导SD大鼠视网膜光感受器细胞凋亡的保护作用。方法将出生后47d的♀SD大鼠随机分为正常对照组、模型组及黄芪3个剂量组,腹腔注射给予生理盐水或黄芪,每日1次,并于给药第4日腹腔注射MNU60mg·kg-1,然后分别于不同时间摘取动物眼球。形态学分析测量视网膜的总厚度;TUNEL法和转录因子分析检测光感受器细胞凋亡和胞核内NFkBp65活性。结果黄芪注射液可显著增加周边视网膜总厚度、降低MNU引起的光感受器细胞凋亡指数,并增加视网膜组织细胞胞核内NFkBp65的活性。结论黄芪注射液对MNU引起的视网膜损伤有一定的保护作用。AIM ：To observe the protective effect of. Astragalus injection on N-methyl-N-nitrosourea （ MNU ）- induced photoreceptor apoptosis in SD rats. METHODS ： Female 47 - day SD rats were randomly divided into control group, model group and three dose groups of Astragalus. Saline or Astragalus was injected intraperitoneally into rats once a day. On 4^th day after treatment, the rats were injected intraperitoneally by 60 mg· kg^-1 MNU and the eyes were taken in different phases. Retinal damage was evaluated based on retinal thickness. The apoptotic index of the photoreceptor cells and the activity of nuclear NF-kB p65 were detected by TUNEL labeling and Transcription Factor Assay Kit, respectively. RESULTS ： Astragalus injection could remarkly increase total thickness of peripheral retina and decrease apoptotic index of photoreceptor cells induced by MNU and increase the activity of nuclear NF-kB p65. CONCLUSION：Astragalus injection partially protects against MNU-induced retinal damage.

关 键 词：N-甲基-N-亚硝脲 黄芪 视网膜 大鼠 

分 类 号：R285.6[医药卫生—中药学]