胆道癌、胆石症患者胆汁标本菌群分析  

Analysis of bacteria in the bile of patients with biliary tract cancer and cholelithiasis

作　　者：胡厚佳[1] 沈思娣 杨梦[1] 程家蓉[3] 高玉堂[3] 洪秀华[1] 

机构地区：[1]上海第二医科大学附属瑞金医院临床医学院,上海200025 [2]上海嘉定区中心医院检验科,上海201800 [3]上海市肿瘤研究所,上海200032

出　　处：《检验医学》2005年第5期459-461,共3页Laboratory Medicine

基　　金：美国国立癌症研究所"胆道癌病例对照研究"科研基金资助项目

摘　　要：目的探讨胆石症发病与细菌感染的关系。方法采用需氧、专性厌氧和微需氧方法,对择期手术治疗的胆道癌、胆石症患者的胆汁标本进行细菌分离培养、API鉴定和菌群分析。结果胆石症患者细菌阳性分离率为36%(34/95),胆石症合并胆道癌患者为43%(6/14),胆道癌患者为57%(8/14);色素型胆石症患者胆汁细菌阳性分离率为77%(10/13),混合型胆石症患者为29%(16/56),胆固醇型胆石症患者为23%(3/13),不明胆石类型胆石症患者为45%(10/22)。共分离出69株菌株,其中肠杆菌科细菌30株,肠球菌属细菌19株,其余为葡萄球菌属细菌、链球菌属细菌等。结论胆石症患者胆汁中分离出细菌,且以色素型胆石症患者胆汁标本细菌阳性分离率最高,胆汁细菌分布又以肠杆菌科细菌和肠球菌属为主,提示色素型胆石症形成与细菌有一定关系。Objective To investigate the relationship between gallstone formation and bacterial infection. Methods Bile was collected aseptically from patients with biliary tract cancer and cholelithiasis. Bile was cultured aerobically, anaerobically and microaerophilically, then API identification and bacteria flora analysis were carried out. Results The positive rate of bacteria in biles of patients with cholelithiasis, cholelithiasis combined with biliary tract cancer and biliary tract cancer was 36% （34/95）, 43% （6/14） and 57% （8/14） respectively. In pigment stone type, mixed gallstone type, cholesterol gallstone type and unknown gallstone type, the positive rate for bacteria was 77% （ 10/13 ） , 29% （ 16/ 56）, 23% （3/13） and 45% （ 10/22 ） respectively. 69 strains of bacteria were detected including 30 strains of Enterobacteriaceae, 19 strains of Enterococcus and others including Staphylococcus, Streptococcus etc. Conclusions Bacteria are isolated in bile of cholelithiasis patients and the positive rate of pigment stone type is the highest. The main kind flo- ra in bile is Enterobacteriaceae and Enterococcus. The results show that pigment stone type formation may be related to bacteria infection.

关 键 词：胆结石 胆汁 细菌 

分 类 号：R446.5[医药卫生—诊断学]