关白附的HPLC指纹图谱  

HPLC Fingerprint of Aconitum coreanum

作　　者：王吓长[1] 刘静涵[2] 王强[1] 

机构地区：[1]中国药科大学中药分析教研室,南京210038 [2]中国药科大学天然药物化学教研室,南京210009

出　　处：《中国天然药物》2005年第5期294-297,共4页

基　　金：国家863项目"创新药物与中药现代化"重大专项资助(No.2002AA223102)~~

摘　　要：目的:对东北产10个批号的关白附药材指纹图谱进行了比较。方法:应用RP-HPLC法,Dia-monsilC18色谱柱,流动相为2mg/mL浓度的庚烷磺酸钠水溶液(含0·2%三乙胺并以H3PO4调pH至3·0)-乙腈二元梯度洗脱,流速为1·0mL/min,检测波长205nm。结果:不同批号的关白附药材所含生物碱成分均得到很好的分离,确定了指纹图谱中10个共有峰,共有峰峰面积之和均大于各总峰面积的90%。结论:该方法可用于关白附的真伪鉴别和质量评价。AIM： To make comparative study on HPLC fingerprint for 10 batches of Aconitum coreanum produced in the Northeast of China. METHOD： Diamonsil C18 column was used with a mobile phase of （A） 2 mg/mL sodium 1-heptanesulfonate （including 0.2% triethylamine and the pH was adjusted to 3.0 with phosphoric acid）-（B）acetonitrile in a gradient mode. The flow rate was 1.0 mL/min. The wavelength of measurement was 205 nm. RESULT： The alkaloid components of Aconitum coreanum from different batch was separated well. There were 10 common peaks in HPLC-FPS, the area summation of which were no less than 90% of the total peak area. CONCLUSION： The method can be used to identify and evaluate the quality of Aconitum coreanum.

关 键 词：关白附 指纹图谱 生物碱 

分 类 号：R917[医药卫生—药物分析学]