电针对大鼠胃肠动力障碍的调整作用及其神经化学机制  

Regulatory Effects of Electroacupuncture of "Zusanli"(ST 36) on Gastrointestinal Motility Disorder in Rats and Its Related Neurochemical Mechanism

作　　者：雷君[1] 李琴[1] 李国成[1] 茹立强[1] 

机构地区：[1]华中科技大学同济医学院神经生物学系,武汉430030

出　　处：《针刺研究》2005年第3期131-137,共7页Acupuncture Research

摘　　要：目的:探讨针刺对胃肠运动功能的调整作用及可能的神经化学机制。方法:Wistar大鼠64只,随机分为正常对照组、胃肠动力障碍组、胃肠动力障碍+西药治疗组和胃肠动力障碍+电针治疗组。采用夹尾刺激激怒法制成大鼠胃肠动力障碍模型,用应力传感器记录大鼠胃和十二指肠消化间期移行性复合运动(MMC),采用组织化学技术观测胃、十二指肠肌间神经丛乙酰胆碱酯酶(AChE)、一氧化氮合酶(NOS)阳性神经元的变化。结果:①MMC的改变:与对照组比较,胃肠动力障碍组胃-肠MMC周期、Ⅰ、Ⅱ相延长,Ⅲ相缩短(P<0.01),Ⅲ相频率减慢、幅度下降(P<0.01),胃Ⅲ相发生率降低(P<0.01),胃-肠平均协调收缩率显著下降(P<0.01);西药治疗组、电针治疗组与胃肠动力障碍组比较,胃-肠MMC周期、Ⅰ、Ⅱ相缩短,Ⅲ相延长(P<0.05,0.01),Ⅲ相频率加快、幅度上升(P<0.01),胃Ⅲ相发生率升高(P<0.01),胃-肠平均协调收缩率显著升高(P<0.01);与西药组比较,电针组各指标的改变较明显(P<0.05,0.01)。②胃窦、十二指肠肌间神经丛AChE阳性神经元表达:与对照组比较,胃肠动力障碍组显著减少(P<0.01);与胃肠动力障碍组比较,西药治疗组、电针治疗组显著增加(P<0.01)。③胃窦、十二指肠肌间神经丛NOS阳性神经元表达:与对照组比较,胃肠动力障碍组显著增加(P<0.01);与胃肠动力障碍组比较,西药治疗组、电针治疗组显著减少(P<0.01)。结论:①胃、十二指肠协调运动障碍可能是胃肠动力障碍大鼠发病机制之一;②胆碱能神经、氮能神经紊乱可能是胃肠动力障碍大鼠胃、十二指肠协调运动障碍的神经化学基础;③针刺能改善胃肠动力障碍大鼠胃、十二指肠运动协调性,效应优于胃肠动力药多潘立酮,其作用机理可能与调节ACh、NO神经递质的释放有关。Objective： To investigate the regulatory effect of electroacupuncture （EA） on gastrointestinal motility and its underlying neurochemical mechanism. Methods： A total of 64 Wistar rats were randomly divided into control, model, medication （Dornperidone, 31.5 mL/kg/d, intragastric perfusion, 7 days altogether） and EA groups, with 16 cases in each group. Gastrointestinal motility disorder model was established by pinching the rat＇s tail repeatedly with a forceps. EA （ 1 - 5 V, 4 - 16 Hz, danse-sparse waves） was applied to bilateral “Zusanli”（ST 36） for 50 min, once daily, one week altogether. The interdigestive migrating motor complex （MMC） waves delivered by using strain gauge electrodes fixed to the subserous layer of gastric antrum and duodenal wall were recorded. Acetylcholinesterase （ACHE） and nitric oxide synthase （NOS） activity in gastroduodanal myenteric plexus was assayed by using ferric cupric cyanide direct coloration method and NADRH-diaphorase method respectively. Resuits： ①MMC： Compared with control group, the cycle, duration of phase Ⅰ and Ⅱ of MMC of thegroup prolonged remarkably （ P 〈 0.01 ）, while the duration, frequency, amplitude and the incidence of phase Ⅲ, and the coordinated contraction rate of the gastric antrum-duodenum in model group all decreased significantly （ P〈0.01 ） ; in comparison with model group, the cycle, duration of phase Ⅰ and Ⅱ of MMC of both stomach and duodenum in medication and EA groups shortened significantly （P〈0.05, 0.01）, while the duration, frequency, amplitude and incidence of phase Ⅲ as well as the coordinated contraction of the stomach-duodenum in medication and EA groups increased significantly （ P〈0.01 ） ; significantly superior to those of medication in the aforementioned indexes （ P〈0.05, 0.01 ）. ② ACHE： Compared with control group, the area density （AD） and optical density （OD） values of myenteric plexus in model group decreased significantly （ P〈 0.01 ） ; w

关 键 词：电针 消化间期移行性复合运动 胃肠动力 AChE和NOS表达 

分 类 号：R245.97[医药卫生—针灸推拿学]