艾灸“肺俞”“膏肓”对博莱霉素A5诱导肺纤维化大鼠Ⅰ/Ⅱ型细胞因子平衡调节的影响  

Influence of Moxibustion of "Feishu"(BL 13) and "Gaohuangshu"(BL 43) on the Regulation of Th1/Th2 Cytokines in BLM_(A5)-induced Pulmonary Fibrosis Rats

作　　者：李戎[1] 周蜜娟[1] 李翠霞[1] 李富红[1] 李文军[1] 蔡永宁[2] 李志刚[3] 罗全安[2] 刘明芳[1] 

机构地区：[1]成都中医药大学,成都610075 [2]四川大学华西医学中心,成都610041 [3]成都理工大学,成都610059

出　　处：《针刺研究》2005年第3期164-166,共3页Acupuncture Research

基　　金：国家自然科学基金项目(No.30472236);国家中医药管理局专项课题(No.04-05JP54)

摘　　要：目的:研究艾灸“肺俞”“膏肓”对博莱霉素(BLM)所致肺纤维化大鼠肺组织Ⅰ/Ⅱ型细胞因子平衡调节的影响,探索针灸阻抑肺纤维化的效应机制。方法:SD大鼠随机分为:空白组、模型组、艾灸组、泼尼松组。气管内注入博莱霉素制作大鼠肺纤维化模型,造模后7 d开始治疗,以5 mg左右艾绒灸其双侧“肺俞”“膏肓”,每穴3壮,每天1次,10 d为1疗程,疗程间休息1 d,3个疗程后处死动物,采用酶联免疫吸附法测定肺组织内Ⅰ型细胞因子IL-2I、L-12和Ⅱ型细胞因子IL-4、IL-5含量。结果:造模后大鼠肺组织内Ⅰ型细胞因子含量降低,Ⅱ型细胞因子含量升高(均P<0.01);艾灸及泼尼松治疗能够使大鼠肺组织内Ⅰ型细胞因子含量升高,Ⅱ型细胞因子含量降低,与模型组比较具有统计学差异(P<0.05,0.01);艾灸组与泼尼松组比较,各指标均无统计学差异(P>0.05)。结论:艾灸“肺俞”“膏肓”能升高BLMA5诱导肺纤维化大鼠肺组织中Ⅰ型细胞因子IL-2I、L-12的含量,降低Ⅱ型细胞因子IL-4I、L-5的含量,提示调节Ⅰ/Ⅱ型细胞因子的平衡可能是艾灸阻抑肺纤维化发生的机理之一。Objectives： To observe the effect of moxibustion of “Feishu”（BL 13） and “Gaohuangshu”（BL 43） on the balance of T helper1/T helper2 （Th1/Th2） cytokines in BLMA5-induced pulmonary fibrosis rats, and to explore its underlying mechanism. Methods： Twenty-four SD rats were evenly randomized into control, model, moxibustion, and medication groups. Pulmonary fibrosis model was established by injection of Bleomycin via trachea （5 mg/kg）. Moxibustion （3 cones/acupoint） was applied to bilateral “Feishu”（BL 13） and “Gaohuangshu”（BL 43）, once daily, with 10 days being a therapeutic course and an interval of one day, 3 courses altogether. Rats of medication group were fed with Prednisone （2.5 mg/kg）, once daily and continuously for 30 days. At the end of experiments, the rats were killed for taking out the left mid-lung tissues to be processed into homogenate samples. Cytokines such as IL-2, IL-12, IL-4 and IL-5 were detected with enzyme linked immunosorbent assay according to instructions of reagent kits. Results： In comparison with control group, IL-2 and IL-12 contents of the lung tissue were significantly lower, while IL-4 and IL-5 considerably higher in model group （ P〈0. 01 ） ; whereas compared with model group, IL-2 and IL-12 contents were significantly higher （ P〈0. 05,0.01 ）, IL-4 and IL-5 levels significantly lower in moxibustion and medication group （ P〈0. 01 ）. No significant differences were found between rnoxibustion group and medication group in the 4 indexes （ P 〉0.05）. It indicated that in BLMA5-induced pulmonary fibrosis rats, Th1 cytokines IL-2 and IL-12 levels in the lung tissue decreased markedly, and Th2 cytokines IL-4 and IL-5 levels increased abnormally, both moxibustion and Prednisone could regulate changes of Thl and Th2 cytokines. Conclusion： Moxibustion of “Feishu”（BL 13） and “Gaohuangshu”（BL 43） can raise the contents of Th1 cytokines （IL-2 and IL-12） and lower those of Th2 cytokines （IL-4 and IL-5

关 键 词：肺间质纤维化 艾灸疗法 Ⅰ型细胞因子 Ⅱ型细胞因子 

分 类 号：R245.81[医药卫生—针灸推拿学]