SPE-MHPLC法测定金银花中绿原酸的研究  

Study on SPE-MHPLC Method for the Determination of Chlorogenic Acidin Flos Lonicerae

作　　者：吴方评[1] 董学畅[1] 张甜[1] 

机构地区：[1]云南民族大学化学与生物技术学院,云南昆明650031

出　　处：《云南民族大学学报（自然科学版）》2005年第4期330-332,共3页Journal of Yunnan Minzu University:Natural Sciences Edition

摘　　要：研究了用固相萃取-微柱高效液相色谱法(SPE-MHPLC)测定金银花中的绿原酸.金银花样品中的绿原酸用80%的甲醇加热回流提取,提取液用Sep-Pak-C18固相萃取小柱预分离脱脂,以W aters X terraTMRP18(1.0×50mm,2.5μm)微柱为固定相,甲醇-乙酸溶液(1%)为流动相,用紫外二级管矩阵检测器检测.回收率在96%-102%之间,RSD在1.5%-2.2%之间.该方法用于金银花样品中绿原酸的测定,结果满意.Solid phase extraction （SPE） and a microcolumn high performance liquid chromatography （MH- PLC） for the determination of chlorogenic acid in Flos lonicerae is studied. The chlorogenic acid can be extracted from the Flos lonicerae sample with 80% of methanol, then subjected to degrease by solid phase extraction with Sep - Pak - C18 cartridge, chlorogenic acid can be separated on a Waters XterraTMRpls （ 1.0 × 50mm, 2.51μm） microcolumn, with methanol and acetic acid solution（ 1% ） as mobile phase and detected with photodiode array detector. Forthermore, The recoveries of tobacco polyphenols are 96% - 102% , and the relative standard deviations are 1.5% -2.2%. This method can be applied to the determination of chlorogenic acid in Flos lonicerae with satisfactory result.

关 键 词：固相萃取 微柱高效液相色谱 金银花 绿原酸 

分 类 号：X830.2[环境科学与工程—环境工程]