二阶导数紫外分光光度法测定酒石酸美托洛尔体外经皮渗透量  

Determination of Transdermal Osmolality of Metoprolol Tartrate by Second-order Derivative UV Spectrophotometry in Vitro

作　　者：王文刚[1] 恽榴红[1] 王睿[2] 周筱青[2] 

机构地区：[1]军事医学科学院毒物药物研究所,北京市100850 [2]解放军总医院临床药理研究室,北京市100853

出　　处：《中国药房》2005年第18期1379-1381,共3页China Pharmacy

摘　　要：目的:建立以二阶导数紫外分光光度法测定酒石酸美托洛尔(MP)体外经皮渗透量的方法.方法:对MP的二阶导数紫外扫描图谱在224nm和234nm波长处出现的特征谷和特征峰间的振幅△A进行定量.结果:MP检测浓度在5～60μg/ml范围内线性关系良好,平均回收率为98.89%,RSD平均值为2.05%.结论:本法可排除皮肤渗出物干扰并准确测定MP体外经皮渗透量,且操作简便、快速,结果准确、可靠.OBJECTIVE： To establish the Second - order Derivative UV - spectrophotometry for the determination of transdermal osmolality of metoprolol tartrate （MP） in vitro. METHODS： The quantitation of the vibration amplitude （△A） between the characteristic valley and the characteristic peak of MP appeared at 224nm and 234nm in UV - scanning. RESULTS： The linear concentration range of metoprolol tartrate was 5～60μg/ml, the average recovery was 98.89% with average RSD at 2.05%. CONCLUSION： This method can accurately determine the transdermal osmolality of MP in vitro yet without the interference of skin exudates, its operation is simple and fast and the results are accurate and reliable.

关 键 词：二阶导数紫外分光光度法 酒石酸美托洛尔 经皮渗透 

分 类 号：R945[医药卫生—微生物与生化药学] R972[医药卫生—药剂学]