中草药抗乙型肝炎病毒活性及其作用机理体外实验研究  

The inhibitory effect of some Chinese herbs on hepatitis B virus replication in vitro and its mechanism

作　　者：范涛[1] 傅希贤[1] 张国庆[1] 张乃临[1] 

机构地区：[1]北京医科大学第一医院传染病教研室,北京100034

出　　处：《中华实验和临床病毒学杂志》1996年第1期27-30,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：用2.2.15细胞株体外抗乙型肝炎病毒(HBV)药物实验模型,对4种中草药制剂体外抗HBV 活性及其机理进行了评价。结果表明:4种中草药对细胞的50%毒性浓度(CD50)分别为乾坤宁>640mg/L,双黄连针剂>640mg/L,复方仙茅浸膏水提取液160g/L(生药计算),复方黄芪浸膏水提取液320g/L(生药计算)。对病毒复制指标 HBsAg、HBeAg 和细胞内 HBV DNA 的50%抑制浓度(ID50)分别为乾坤宁58.9mg/L,145.9mg/L 和71.01mg/L。双黄连针剂98mg/L,206.5mg/L 和18.12mg/L。复方仙茅浸膏水提取液30g/L,70g/L 和25.83g/L。复方黄芪浸膏水提取液的 HBsAg 和 HBeAg ID50分别为60g/L 和110g/L,但对 HBV DNA 无明显抑制,HBVDNA ID50>160g/L,提示该药可能作用于病毒的转录、翻译和蛋白合成等环节或直接与病毒蛋白结合而致 HBsAg、HBeAg 滴度下降。Using an assay system based on 2.2.15 cell line,some Chinese herbs were studied for their inhibitory ability on hepatitis B virus replication and its mechanism.The results show that the concentrations for 50% cell viability(CD50)were>640mg/L Qiankunning or Shuanghuanglian,160g/L Rhizoma Culiginis.Co.,320g/L Radix Astragali.Co..The concentrations for 50% inhibition of HBsAg/HBeAg expression and HBV DNA replication(ID50)were 58.9mg/L,145.9mg/L and 71.01mg/L with Qiankunning,98mg/L,206.5mg/L and 18.12mg/L with Shuanghuanglian,30g/L,70g/L and 25.83g/L with Rhizoma Curculiginis.Co.,respectively. Radix Astragali.Co.show remarkable inhibitory effect on viral protein expression(ID50 of HBsAg and HBeAg were 60g/L and 110g/L),however,it show low effect on HBV DNA synthesis,even at the concentration of 160g/L,the results suggest that it is likely to affect HBV multiplication in other stages of replication such as viral RNA synthesis and/or viral protein synthesis.

关 键 词：细胞株 乙型肝炎病毒 中草药 DNA 病毒 

分 类 号：R259.126[医药卫生—中西医结合] R285.5[医药卫生—中医内科学]