麝香草酚和香荆芥酚对痢疾杆菌和肠炎常见菌的体外抗菌效应  

In Vitro Antibacterial Effects of Thymol and Carvacrol on Dysentery Bacteria and Common Bacteria Causing Enteritis

作　　者：廖芳[1] 杨振德[1] 黄庆华[1] 许汉林[2] 徐可树[3] 

机构地区：[1]华中科技大学同济医学院基础医学院病原生物学系,武汉430030 [2]湖北中医学院药学系,武汉430061 [3]华中科技大学同济医学院附属协和医院消化科,武汉430022

出　　处：《医药导报》2005年第10期868-870,共3页Herald of Medicine

摘　　要：目的研究麝香草酚和香荆芥酚对痢疾杆菌和肠炎常见菌的体外抗菌作用,证实此两种物质为牛至的主要有效抗菌成分.方法采用稀释法体外测试麝香草酚和香荆芥酚对痢疾杆菌4菌株和致病性大肠埃希菌等71个菌株的最低抑菌浓度(MIC)和最低杀菌浓度(MBC).结果麝香草酚对痢疾杆菌的MIC为0.125～0.250 mg·mL-1,MBC为0.250～0.500 mg·mL-1;对71株肠炎常见菌的MIC为0.031～0.500 mg·mL-1,MBC为0.062～1.000mg·mL-1.香荆芥酚对痢疾杆菌的MIC为0.125～0.500 mg·mL-1,MBC为0.250～1.000 mg·mL-1;对71株肠炎常见菌的MIC为0.062～0.500 mg·mL-1,MBC为0.125～1.000 mg·mL-1.结论麝香草酚和香荆芥酚是牛至的主要有效抗菌成分,体外对痢疾杆菌和肠炎常见菌均具有较强的抑菌和杀菌作用.Objective To study the antibacterial effects of thymol and carvacrol on dysentery bacteria and common bacteria causing enteritis in vitro. Methods The minimal inhibitory concentration （MIC） and minimal bactericidal concentration （MBC） of thymol and carvacrol for dysentery bacteria （4 serum types） and 71 strains of pathogenic Escherichia coli and other bacteria causing enteritis were determined in vitro with the dilution method. Results The MIC and MBC of thymol for dysentery bacteria were 0.125-0.250 mg· mL^-1 and 0.250-0.500 mg·mL^-1, respectively. The MIC and MBC of thymol for the common bacteria causing enteritis were 0.031-0. 500 mg·mL^-1 and 0.062-1.000 mg·mL^-1 , respectively. The MIC and MBC of carvacrol for dysentery bacteria were 0.125-0.500 mg·mL^-1 and 0.250-1.000 mg·mL^-1, respectively. The MIC and MBC of carvacrol for the common bacteria causing enteritis were 0.062-0.500 mg·mL^-1 and 0.125-1.000 mg·mL^-1, respectively. Conclusion Thymol and carvacrol were shown to be the major antibacterial components of origanum vulgare, These 2 substances exerted fairly strong bacteriostatic and bactericial effects on dysentery bacteria and common bacteria causing enteritis in vitro.

关 键 词：麝香草酚 香荆芥酚 最低抑菌浓度 最低杀菌浓度 

分 类 号：R284[医药卫生—中药学] R285.5[医药卫生—中医学]