吸入性糖皮质激素对哮喘小鼠核因子-κB及Th2类细胞因子的影响  

Influence of Inhaled Glucocorticoids on Nuclear Factor - KB and Th2 Cytokines of Murine with Asthma

作　　者：孔春妍[1] 张岩[2] 王明月[1] 王岷[1] 刘艳苹[1] 张萍[1] 

机构地区：[1]济南铁路中心医院 [2]山东大学齐鲁医院,济南250001

出　　处：《实用儿科临床杂志》2005年第10期974-975,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨糖皮质激素对哮喘小鼠核因子一.cB(NI:}一.cB)表达及Th2类细胞因子的影响。方法30只BAL.B/c小鼠随机分为正常组、哮喘组、布地奈德治疗组,采用ELISA法测定其支气管肺泡灌洗液(BA[廖)中IL.4、IL_5、IFN一_y的含量,免疫组织化学检测肺组织NF-|cB表达。结果哮喘组与正常组、治疗组比较肺组织NF:g13表达及BAL.F中IL.4、IL.5含量均有显著差异(P均<O.01)。Nf?。g13活化细胞百分比与BAIJ中IL一4、IL5含量呈显著正相关(r=0.79.O.68P<O.01)。结论糖皮质激素抑制肺组织NF_.cEj激活。NF-rJ3与IL-4、IL-5的产生相关,糖皮质激素通过抑制1112类细胞功能来增强Thl反应。Objective To explore the effect of glucocorticoids on nuclear factor- kB expression and Th2 cytokines and the relationship of these two factors. Metlods Thirty BALB/c mice were divided randomly into normal control groups, asthmatic groups and budesonide inhalation group. The expression of NF - kB was observed by immunocytochnfical staining; IL - 4, IL - 5, IFN - γ proteins in BALF were measured by ELISA. Results The percentage of cells of active NF - kB, IL - 4 and IL - 5 protein in BALF of asthmatic group was significantly higher than that of control group,which was significantly reduced by budesonide（P〈0.01 ）. There was good positive correlation between the percentage of cells of active NF -kB and the degree of IL-4, IL - 5 protein in BALF（ r = 0.79,0.68 P 〈0.01）. Conclusions Glucocorticoids may reduce the activation of NF- kB. The activation of NF - kB is related to the production of IL-4, IL - 5 protein. Glucocorticoids can restrain the function of Th2 cells.

关 键 词：糖皮质激素类 核因子-KB 哮喘 白细胞介素 小鼠 

分 类 号：R96[医药卫生—药理学]