汞电极表面痕量DNA的电化学响应及其检测(英文)  

Current Response and Determination of Trace DNA at the Mercury Electrode

作　　者：韩君[1] 史海滨[1] 安■顺一 陈强[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]日本东北大学药学研究科

出　　处：《南开大学学报（自然科学版）》2005年第3期93-96,共4页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：SupportedbyScienceFoundationofTianjin(033802711)

摘　　要：用电化学方法来研究在汞电极上的DNA样本并得到了一个较为理想的DNA的响应电流.实验结果表明,尽管不同DNA样本在检测结果上有着微小的差异,但它们都证实这种电化学方法可作为一种新的系统性的分析痕量DNA方法.因此,可以发展一种由电化学方法促进的极谱法来检测痕量的DNA.在最适条件下,以这种方法检测DNA的浓度范围在2～20 μg/mL内呈良好的线性关系.检测极限为1.0μg/mL.相对标准偏差为4.5%(10μg/mL DNA,6次连续检测).鉴于电化学技术的优点,这一方法可能在生物化学和分子生物学领域发挥作用.DNA samples were studied using electrochemical methods and a useful current response of DNA at mercury electrodes was obtained. Experimental results revealed that various samples gave slight different results, but they all exhibit the suitability necessary for a methodical analysis. Accordingly, a polarographic method was developed with an electrochemical technique for the determination trace DNA. Under optimum conditions, the proposed method provided a linear response DNA concentrations in the range of 2-20μg/mL. The detection limit was 1.0μg/mL. The relative standard deviation was 4.5 % for 6 successive determinations of DNA at 10μg/mL, this method may be of considerable value in both the biochemical and molecular biological fields.

关 键 词：DNA 极谱法 检测 

分 类 号：Q657.1[生物学—生物物理学]