样品堆积富集毛细管电泳测定磺化杯芳烃  

DETERMINATION OF SULFONATED CALIXARENES BY CAPILLARY ELECTROPHORESIS WITH SAMPLE STACKING ENRICHMENT

作　　者：傅崇岗[1] 刘爱林[1] 赵艳芳[1] 张立云[1] 

机构地区：[1]聊城大学化学化工学院,聊城252059

出　　处：《理化检验（化学分册）》2005年第9期658-660,共3页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：山东省青年科学基金(Q99B11);聊城大学科研基金项目

摘　　要：利用毛细管电泳技术,建立了一种分离、测定磺化杯芳烃的方法.研究了缓冲液浓度和pH、分离电压和进样时间等因素对分离性能的影响.采用磷酸缓冲液(pH 8.7)作分离介质,分离电压15 kV时,6 min内可实现对磺化杯[4]芳烃、磺化杯[6]芳烃和磺化杯[8]芳烃的完全分离.该法充分利用了样品堆积效应,无需任何添加剂,与文献相比简单快速.磺化杯芳烃的浓度(0.001 0～1.0 mmol·L-1)与峰高呈良好的线性关系,其相关系数均优于0.999 3,检出限分别为0.43,0.25和0.32μmol·L-1.A new method based on capillary electrophoresis was developed for the separation and determination of sulfonated calixarenes. Effects of several important factors such as the pH and concentration of buffer, separation voltage and sample injection time were investigated to optimize the conditions. Three sulfonated calixarenes were well separated within 6 min in a 37 cm length capillary at a separation voltage of 15 kV in a 10 mmol·L^-1 phosphate buffer （pH 8.7）. Making full use of sample stacking effect, additives were not necessary in this method thus making it is much simpler and faster than that reported in literature. The relationship of peak heights and concentrations was linear over about three orders of magnitude with correlation coefficients over 0. 999 3 and detection limits （S/N=3） of 0. 43, 0. 25 and 0. 32 μmol · L^-1 for 3 sulfonated calixarenes i. e. sulfonated calixarene[4], sulfonated calixarene[6] and sulfonated calixarene[8], respectively.

关 键 词：毛细管电泳 超分子 样品堆积 磺化杯芳烃 

分 类 号：O657[理学—分析化学]