细胞因子组合对受照射脐带血AC133^+细胞周期和早期凋亡的影响  

EFFECTS OF CYTOKINE COMBINATIONS ON THE CELL CYCLE AND EARLY APOPTOSIS OF IRRADIATED UMBILICAL CORD BLOOD AC133^+ CELLS

作　　者：刘玉龙[1] 戴宏[1] 姜忠[1] 周丽英[1] 郭晓葵[1] 周剑影[1] 胡勤芳[2] 

机构地区：[1]苏州大学附属第二医院中国核工业辐射损伤医学应急中心,215004 [2]北京核工业医院,100081

出　　处：《辐射防护》2005年第5期311-315,共5页Radiation Protection

基　　金：国防科工委"十五"国防预研"急性放射损伤救治技术研究"(Y-020403-QS)资助项目。

摘　　要：采用免疫标记法,使用流式细胞仪测定受到2.5Gy6MV-X射线照射的脐带血AC133+细胞加细胞因子组合(IL-3+FL+SCF)在培养不同天数后的细胞周期及早期凋亡情况的变化。结果表明,新鲜分离的未受照的脐带血AC133+细胞在无细胞因子的短期液体培养体系中培养2天,99%的细胞处于G0/G1期;受照的脐带血AC133+细胞加细胞因子组合(IL-3+FL+SCF)培养不同天数后,脐带血AC133+细胞迅速进入增殖循环,在照后第3天,有近50%的细胞进入S期。脐带血AC133+细胞受照后不加细胞因子,培养48小时后,镜下观察到细胞全部死亡;加细胞因子组合(IL-3+FL+SCF)培养48小时后,有(38.0±6.8)%的受照的脐带血AC133+细胞被“救活”,且随着培养时间的延长,被“救活”的AC133+细胞又出现了增殖。因此,可认为细胞因子组合(IL-3+FL+SCF)对受照的脐带血AC133+细胞具有保护作用。The cell cycle and early apoptosis of 2.5 Gy 6 MV-X my irradiated umbilical cord blood AC133^＋ cells cultured with cytokine combinations （IL-3 ＋ FL ＋ SCF） were immunolabelled and analyzed by flow cytometry at d O, 1, 2, 3 and 7. The result of flow cytometry analysis showed that majority of irradiated umbilical cord blood AC133^＋ cells were in G0/G1 phase of the cell cycle at d O. Under the influence of cytokine cembinations （IL-3 ＋ FL＋ SCF）, nearly 50% of cells were in S phase on 3rd day. AC133^＋ cells irradiated were in vitro incubated in the medium without cytokines, nearly all cells died by apoptosis. However, when we incubated cells with cytokine combinations （IL-3 ＋ FL ＋ SCF）, （38.0 ± 6.8）% of cells were saved from apoptosis at d 2. The more percent of saved AC133^ ＋ cells became to proliferate with the extention of culture. In short, cytokine combinations （IL-3 ＋FL ＋ SCF） could have a key role to protect irradiated cells and partially avoid induction of apoptosis by ionizing radiation in hematopoietics stem/ progenitor cells.

关 键 词：脐带血 AC133^+细胞 电离辐射 细胞因子 细胞凋亡 细胞周期 细胞周期 细胞因子 X射线照射 脐带血 凋亡 早期 液体培养体系 流式细胞仪 镜下观察 

分 类 号：R392[医药卫生—免疫学]