厦门港浮游动物体内色氨酸的荧光法测定  

Measurement of the Content of Tryptophan for Zooplankton from Xiamen Harbour with Fluorescence Method

作　　者：张润[1] 郭卫东[1] 郭东晖[1] 吴芳[1] 夏恩琴[1] 吴鼎勋[1] 

机构地区：[1]厦门大学海洋学系,亚热带海洋研究所,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2005年第5期738-740,共3页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金(40106007);福建省自然科学基金(B9910004)资助

摘　　要：建立了海洋浮游动物体内色氨酸的荧光分析方法.将1 mg左右样品置于安瓿瓶中,加1 mL 5.5 mol/L NaOH为水解液,在110℃水解20 h.水解产物用HCl中和,然后用KH2PO4-NaOH缓冲液调节pH为10.5,在激发波长225 nm,发射波长350 nm处测定荧光强度.方法线性范围0～0.07 mg/L,检测限为2.5×10-3 mg/L,回收率99.5%～ 109.3%.应用该方法测量了7种从厦门港采集的浮游动物的色氨酸含量.A sensitive method for rapid determination of tryptophan contents in zooplankton samples by spectrofluorimetry was proposed. Seven zooplankton samples were collected from the Jiulong River Estuary of Xiamen Harbour at night by plankton net from March 2004 to May 2004. Each single species were picked out under microscope at the laboratory. They were immediately dried to constant weight at 60℃ for 48 h and were grinded into powder before analysis. 1 mg of zooplankton sample was placed into the ampoule-bottle for hydrolysis. 1 mL of solution of 5.5 mol/L NaOH containing 0. 5% soluble starch was added as hydrolytic solution. Hydrolyzate was neutralized with 6mol/L after hydrolysis at 110℃ for 20 h. And then it was adjusted to pH=10.5 with the buffer KH2PO4-NaOH. The fluorescence intensity was determined at excitation/emission wavelength 225 nm/350 nm. The linear range of calibration graph was 0-0.07 mg/L. The detection limit was 2.5 × 10^-2 mg/L. The recovery rate of sample addition experiments was 99. 5%-109. 3%. The contents of tryptophan in seven zooplankton samples were 8 750 mg·g^-1 （Sagitta bedoti）,7 070 mg · g^-1 （Centropages tenuiremis）,2 890 mg·g^-1 （Corophium sinensis）,4 960 mg · g^-1 （Pseudeuphausia sinica）,6 770 mg · g^-1 （postlarva of Acetes japonicus）,1 850 mg · g^-2 （zoea of Brachyura） and 5 510 mg·g^-1 （megalopa of Brachyura）,respectively.

关 键 词：荧光法 色氨酸 浮游动物 厦门港 

分 类 号：Q503[生物学—生物化学]