应用高效液相色谱-二极管阵列检测器-蒸发光散射检测器联用技术同时测定清开灵注射液中的五类有效成分  

Simultaneous Determination of Five Groups of Componentsin Qingkailing Injection by High Performance LiquidChromatography with Photo Diode Array Detectorand Evaporative Light Scattering Detector

作　　者：严诗楷[1] 辛文峰[1] 罗国安[1] 王义明[1] 程翼宇[2] 

机构地区：[1]清华大学化学系,北京100084 [2]浙江大学药学院中药科学与工程学系,浙江杭州310027

出　　处：《色谱》2005年第5期482-486,共5页Chinese Journal of Chromatography

基　　金：国家重点基础研究发展规划项目(国家"973"研究项目)(No.G1999054404);科技部重大专项基金(No.2002BA906A29-3)资助项目

摘　　要：建立了复方清开灵注射液中5类主要有效成分的定量测定方法。应用高效液相色谱-二极管阵列检测器-蒸发光散射检测器联用技术(HPLC-DAD-ELSD),根据各类成分紫外吸收光谱的差异,分别在240,254,280和330nm波长下检测栀子苷、核苷(包括尿苷和腺苷)、黄芩苷和有机酸(包括绿原酸和咖啡酸)等4类成分,同时使用ELSD测定胆酸、熊去氧胆酸和猪去氧胆酸等3种甾体化合物,从而实现了清开灵注射液中5类有效成分(共9个化合物)的同时分离和定量测定。用该法测定了3个不同厂家的19批清开灵注射液成品。该法快速、准确,操作相对简单,为中药复方复杂体系的多组分定量测定和质量控制提供了一种可靠、合理且简便、易行的方法模式。A method was established for the simultaneous quantification of nine components of five different structural types in Qingkailing injection. High performance liquid chromatography coupled with a photo diode array detector and an evaporative light scattering detector （ HPLC- DAD-ELSD） was employed in the determination. Four monitoring wavelengths of 240, 254, 280 and 330 nm were set to determine nucleosides （uridine and adenosine）, iridoid glucoside （geniposide）, flavone glycoside （baicalin） and organic acids （chlorogenic acid and caffeic acid） respectively, and a combined evaporative light scattering detector was used to detect three steroid compounds （cholic acid, ursodesoxycholic acid and hyodeoxycholic acid）. The proposed method permitted the simultaneous separation and determination of five groups of compounds in Qingkailing injection, and acceptable validation results of the precision, repeatability, stability and accuracy tests were achieved. The method was applied to the analysis of 19 Qingkailing injection samples from three different plants, and the results indicated that the method could be used as a convenient and reliable method in the multi-component determination and quality control of traditional Chinese medicines.

关 键 词：高效液相色谱法 二极管阵列检测器 蒸发光散射检测器 有效成分 清开灵注射液 

分 类 号：O658[理学—分析化学]