CCR5△32、CCR5m303、CCR264I、SDF13′A基因多态性对中国HIV-1感染者预后的影响  

Effect of CCR5△32, CCR5m303, CCR2-64I and SDF1-3′A gene polymorphism on the prognosis of Chinese HIV-1 carriers

作　　者：王晓辉[1] 冯铁建[1] 王福生[2] 陈琳[1] 石向东[1] 金磊[2] 

机构地区：[1]深圳市疾病预防控制中心艾滋病防治科,518020 [2]解放军第三○二医院传染病研究所

出　　处：《中华实验和临床病毒学杂志》2005年第3期256-259,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：广东省卫生厅基金资助(A2001638)

摘　　要：目的研究CCR5△32、CCR5m303、CCR264I、SDF13′A基因多态性对中国HIV1感染者预后的影响。方法对在深圳地区发现的HIV1感染者进行流行病学调查,应用PCR/RFLP技术分析感染者CCR5△32、CCR5m303、CCR264I、SDF13′A4种基因的多态性,对部分人群进行HIV1血浆病毒载量和CD4+细胞计数的检测,判断感染者的潜伏期,使用SPSS11.0统计软件分析基因多态性对感染者病毒载量和潜伏期的影响。结果在189例HIV1感染者中没有发现CCR5△32和CCR5m303突变基因型,SDF13′A的等位基因频率为26.14%,CCR264I的等位基因频率为19.82%。方差分析发现,CCR264I基因野生型与杂合型的两组HIV1感染者人群病毒载量对数的大小差异无统计学意义(P=0.272),两组人群潜伏期的长短差异无统计学意义(P=0.662)。SDF1基因野生型、杂合型和纯合型的3组HIV1感染者人群病毒载量对数的大小差异有统计学意义(P=0.001),但3组人群潜伏期的长短差异无统计学意义(P=0.228)。结论CCR264I基因突变对中国汉族HIV1感染者病毒载量没有明显影响,因而也不影响感染者的潜伏期。SDF13′A基因突变对于病毒载量有降低作用,但对延长感染者潜伏期可能没有作用。Objective To study how CCR5 △32, CCR5m303, CCR2-641, SDF1-3＇A gene polymorphisms affect the prognosis of Chinese HIV-1 carrier. Methods Epidemiologie survey was done to the HIV-1 carriers who were found in Shenzhen area. PCR/RFLP technology was applied to analyze CCR5 A32, CCR5m303, CCR2-641, SDF1-3＇A gene polymorphisms of the HIV-1 carriers. The plasma virus load and CD4^＋ cell counting was assayed. The ineubation period of some carriers was estimated. SPSS11.0 software was used to analyze the data. Results No persons with CCR5 A32 and CCR5m303 mutation genotype were found from 189 HIV-1 carriers. SDF1-3＇A allele frequency was 26. 14% and CCR2-641 allele 19.82%. The carriers were divided into high virus load group（ virus load≥20 000 eopies/ml） and low virus load group（virus load 〈20 000 copies/ml）. It was found b） one-way ANOVA analysis on the logarithm of virus load that there was no significant difference between CCR2-64I wild genotype and cross bred genotype （P = 0. 272）. One-way ANOVA analysis on delitescenee of some earriers showed that there was not significant difference between CCR2-64I wild genotype and cross bred genotype （ P = 0. 662） . One-way ANOVA analysis on the logarithm of virus load showed that there was significant difference among SDF1-3＇A wild genotype, cross bred genotype and pure mutation genotype （P =0. 001 ）. Conclusion CCR2-64I gene mutation may not significantly affect virus load of Chinese HIV-1 carriers, nor it affect the incubation period of HIV-1 carriers. SDF1-3＇A gene mutation can decrease virus load, but it may not prolong the incubation period of HIV-1 carriers.

关 键 词：HIV-1 遗传学技术 基因频率 预后 HIV-1感染者 CCR2-64I 基因多态性 SDF1 中国汉族 CCR5△32 

分 类 号：R512.91[医药卫生—内科学] R512.910.2[医药卫生—临床医学]