兔斑块内血管生成与动脉粥样硬化形成和发展关系的探讨  

The connection of plaque angiogenesis and progression of atherosclerotic plaque in rabbites

作　　者：肖敏[1] 吕军[1] 陈立东[1] 刘峰松[1] 张群林[1] 黄从新[2] 

机构地区：[1]郧阳医学院附属太和医院急诊科,湖北十堰442000 [2]武汉大学人民医院

出　　处：《临床心血管病杂志》2005年第9期548-551,共4页Journal of Clinical Cardiology

基　　金：湖北省教育厅科研基金资助项目(No:2002A3008)

摘　　要：目的:观察兔斑块内血管内皮生长因子(VEGF)与动脉粥样硬化(AS)斑块形成和发展的关系。方法:15只日本大耳白兔随机分为3组:对照组用普通饲料喂养,高脂组及VFGF组给高胆固醇饲料喂养3周(21 d)后,对照组及高脂组肌内注射清蛋白2μg/kg,VEGF组肌内注射VEGF165 2μg/kg,继续以前方式饲养3周(共42 d)处死动物,截取胸主动脉进行计量组织学及免疫组织化学分析,测定不同组别不同时间点兔血清白细胞介素-8(IL-8)浓度和血脂浓度。结果:①斑块面积(PA)[对照组0,高脂组(1.81±0.61)%,VEGF组(24.12±3.58)%]、斑块周径[对照组0,高脂组(6.05±1.62)%,VEGF组(25.71±2.97)%]及斑块的最大厚度[对照组0,高脂组(0.06±0.002)mm,VEGF组(0.16±0.007)mm],各组间比较差异有统计学意义(P<0.05);②42 d 时,新生血管的密度对照组、高脂组、VEGF组之间比较差异有统计学意义(P<0.05);③电镜显示:新生血管与AS斑块相邻,新生血管腔内可见淋巴细胞。④VEGF组CD34阳性细胞数与PA之间呈正相关(r=0.989,P< 0.01)。⑤血清IL-8浓度各组间比较差异有统计学意义,但血清TC浓度高脂组与VEGF组差异无统计学意义(P>0.05)。结论:斑块内血管生成是AS斑块的重要病理特征,该过程可能与炎性反应有关。Objective： To evaluate the connection of plaque angiogenesis and progression of atheroselerotie plaque. Method：Fifteen rabbits were randomly divided into three groups , control group （ n =5） fed with common diet and other groups（high cholesterol group, n=5; VEGFgroup, n =5） fed with high cholesterol diet. Albumin or VEGF165 was administered by a single-intramuscular injection （2 μg/kg） to rabbits fed with cholesterol diet or common diet at beginning 3 weeks before therapy. Subsets of rabbits from each group were underwent perfusion fixation and harvesting of the thoracic aorta for morphometric and immunohistochemical analysis at 42 days. Moreover, the changes of serum level of IL -8 and TC in the corresponding time points and groups were determined as well. Result： ①Significant difference was shown in comparing mean plaque area[（control-group 0 % ,high cholesterol group（1.81±0.61）%,VEGF-group （24. 12±3.58） %）], plaque circumference [Control group 0,high-cholesterol-group （6.05±1.62） %, VEGF-group （25.71±2.97） %] and maximal plaque thickness（control-group 0, high cholesterol group （0. 06±0. 002）mm,VEGP group （0. 16±0. 007） mm] respectively. ②There were signifi cant differences in neovascularization density [number of CD34 positive cells （cells/mm^2） control group 0, high cholesterol group （12.35±2.02） %,VEGF-group（61. 15±7.55）% , P〈0.05）2 in control- group, high cholesterol groups and VEGF group at 42 day. ③TEM（transmission electron microscopy ）image was shown intimal vessels associated with lesion and the capillary lumen contain lymphocytes. ④There was positive correlation of Neovascularization （CD34 positive area ）with plaque area（ r =0. 989, P 〈0.01）in VEGF-group at 42 day.⑤ Signifi cant difference was shown in comparing levels of serum IL-8 in the corresponding time points and groups, but there is no significant difference in comparing levels of serum TC in the corresponding time points and groups. Conclusion：Pla

关 键 词：动脉粥样硬化 血管内皮生长因子 血管生成 白细胞介素-8 

分 类 号：R543.5[医药卫生—心血管疾病]