CD4^+CD25^+T细胞在CD8^+T细胞抗肿瘤免疫中的调节作用  

The regulatory role of CD4^+CD25^+ T cells in the CD8^+ T cells mediated anti-tumor immunity

作　　者：施敏敏[1] 刘炳亚[1] 李强[1] 张轶[1] 陈雪华[1] 陈皓[1] 朱正纲[1] 

机构地区：[1]上海第二医科大学附属瑞金医院外科上海消化外科研究所,上海200025

出　　处：《现代免疫学》2005年第5期384-388,共5页Current Immunology

基　　金：国家自然科学基金资助项目(30170915)

摘　　要：实验旨在研究CD4+CD25+T细胞在CD8+T细胞抗肿瘤免疫中的调节作用。将小鼠脾脏中分离的单个核细胞分为两组,即去除CD4+CD25+T细胞组和未去除CD4+CD25+T细胞组,测定树突状细胞提呈的肿瘤抗原多肽刺激不同T细胞增殖活性、细胞因子IFN-γ分泌,以及多肽特异性CD8+T细胞对同源性胃癌细胞株MFC的杀伤活性。结果显示预先去除未致敏T细胞中的CD4+CD25+T细胞,所诱导的特异性CD8+CTL对肿瘤细胞免疫应答增强,表现为反应性T细胞对树突状细胞提呈的肿瘤抗原多肽增殖反应增强,IFN-γ分泌量提高及CD8+T细胞对MFC杀伤活性增强。这些结果表明,预先去除未致敏T细胞中的CD4+CD25+T细胞,肿瘤抗原多肽修饰的树突状细胞肿瘤疫苗效能可明显增加。CD4+CD25+T细胞在CD8+T细胞抗肿瘤免疫中起下调作用。The regulatory role of CD4^＋CD25^＋T cells in the CD8^＋T cell mediated anti-tumor immunity was investigated in the present study, in which the mononueleated cells （MNC） isolated from mouse spleen were divided into the CD4^＋CD25^＋ T cells eliminated group of mice and that without elimination of cells, and the proliferative activity of T lymphoeytes induced by peptides of tumor antigens presented through dendritic cells, release of IFN-γ and the killing activity of peptide-specific CDS＋T cells to the homologous gastric carcinoma cell line MFC were assayed in vitro, respectively. The experimental results demonstrated that the CDS＋T cells in group of mice with elimination of CD4^＋CD25^＋T cells could induce enhanced anti-tumor immunity in terms of stronger proliferative activity of T lymphocytes against peptide pulsed dendritic cells, elevation of IFN-γ secretion as well as enhanced cell lysis against homologous cancer cells. It is concluded that the elimination of the CD4^＋CD25^＋T cells in the nalve T cells population can significantly improve the potency of peptide augmented dendritic cells based tumor vaccine, and the CD4^＋CD25^＋T cells can down-regulate the CD8^＋ T cell mediated anti-tumor immunity.

关 键 词：CD4^+CD25^+T细胞 多肽表位 树突状细胞 肿瘤 免疫治疗 

分 类 号：R392[医药卫生—免疫学] R730.54[医药卫生—基础医学]